Jamshidi Mitra, Ghanati Faezeh
Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran.
Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran.
Plant Physiol Biochem. 2017 Jan;110:178-184. doi: 10.1016/j.plaphy.2016.04.026. Epub 2016 Apr 16.
The toxicity of silver nanoparticles (AgNPs) has been attributed to the generation of Ag ions as well as production of ROS. The latter can elicit defensive response of plant cells in different ways e.g., enhancement of secondary metabolite productions. In the present study this hypothesis was evaluated by assessment of taxanes production by suspension-cultured hazel (Corylus avellana L.) cells after treatment with AgNPs. The cells were treated with different concentrations of AgNPs (0, 2.5, 5, and 10 ppm), in their logarithmic growth phase (d7) and were harvested after 1 weak. The growth of cells and their membrane integrity decreased but extracellular electro conductivity and total dissolved solids increase by AgNPs (probably due to loosening of cell membrane). Treatment of hazel cells with AgNPs (in particular of 5 ppm) rapidly and remarkably increased the yields of two major taxanes, i.e., Taxol and baccatin III; so that 24 h of the treatment their contents reached to 378% and 163% of the control, respectively. Increase of Taxanes was accompanied by the increase of total soluble phenols. The extracts of AgNPs-treated cells were able to inhibit the growth of cancerous HeLa cells and reduce their viability to 60% of the control. The results suggest the elicitation of suspension-cultured hazel cells with AgNPs as a procedure for rapid enhancement of anticancer taxanes biosynthesis by the cells.
银纳米颗粒（AgNPs）的毒性归因于银离子的产生以及活性氧（ROS）的生成。后者可以通过不同方式引发植物细胞的防御反应，例如增强次生代谢产物的产生。在本研究中，通过评估悬浮培养的榛树（欧洲榛，Corylus avellana L.）细胞在经AgNPs处理后紫杉烷的产生情况，对这一假设进行了评估。在对数生长期（第7天）用不同浓度（0、2.5、5和10 ppm）的AgNPs处理细胞，并在1周后收获。细胞的生长及其膜完整性下降，但AgNPs使细胞外电导率和总溶解固体增加（可能是由于细胞膜松弛）。用AgNPs处理榛树细胞（特别是5 ppm的处理）迅速且显著地提高了两种主要紫杉烷即紫杉醇和巴卡亭III的产量；以至于在处理24小时后，它们的含量分别达到对照的378%和163%。紫杉烷的增加伴随着总可溶性酚的增加。经AgNPs处理的细胞提取物能够抑制癌细胞HeLa细胞的生长，并将其活力降低至对照的60%。结果表明，用AgNPs激发悬浮培养的榛树细胞是一种快速增强细胞抗癌紫杉烷生物合成的方法。
