Suppr超能文献

利用链特异性深度测序对小非编码RNA进行大规模鉴定及对羊种布鲁氏菌中一种新型毒力相关小RNA的表征

Large-scale identification of small noncoding RNA with strand-specific deep sequencing and characterization of a novel virulence-related sRNA in Brucella melitensis.

作者信息

Zhong Zhijun, Xu Xiaoyang, Li Xinran, Liu Shiwei, Lei Shuangshuang, Yang Mingjuan, Yu Jiuxuan, Yuan Jiuyun, Ke Yuehua, Du Xinying, Wang Zhoujia, Ren Zhihua, Peng Guangneng, Wang Yufei, Chen Zeliang

机构信息

College of Veterinary Medicine, Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Sichuan Province, Chengdu 611130, P. R. China.

Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, P. R. China.

出版信息

Sci Rep. 2016 Apr 26;6:25123. doi: 10.1038/srep25123.

Abstract

Brucella is the causative agent of brucellosis, a worldwide epidemic zoonosis. Small noncoding RNAs (sRNAs) are important modulators of gene expression and involved in pathogenesis and stress adaptation of Brucella. In this study, using a strand-specific RNA deep-sequencing approach, we identified a global set of sRNAs expressed by B. melitensis 16M. In total, 1321 sRNAs were identified, ranging from 100 to 600 nucleotides. These sRNAs differ in their expression levels and strand and chromosomal distributions. The role of BSR0441, one of these sRNAs, in the virulence of B. melitensis 16M was further characterized. BSR0441 was highly induced during the infection of macrophages and mice. The deletion mutant of BSR0441 showed significantly reduced spleen colonization in the middle and late phases of infection. The expression of the BSR0441 target mRNA genes was also altered in the BSR0441 mutant strain during macrophage and mice infection, which is consistent with its reduced intracellular survival capacity. In summary, Brucella encodes a large number of sRNAs, which may be involved in the stress adaptation and virulence of Brucella. Further investigation of these regulators will extend our understanding of the Brucella pathogenesis mechanism and the interactions between Brucella and its hosts.

摘要

布鲁氏菌是布鲁氏菌病的病原体,布鲁氏菌病是一种全球性流行的人畜共患病。小非编码RNA(sRNA)是基因表达的重要调节因子,参与布鲁氏菌的发病机制和应激适应。在本研究中,我们采用链特异性RNA深度测序方法,鉴定了由羊种布鲁氏菌16M表达的一组全局sRNA。总共鉴定出1321个sRNA,长度从100到600个核苷酸不等。这些sRNA在表达水平、链和染色体分布上存在差异。其中一个sRNA,即BSR0441,在羊种布鲁氏菌16M毒力中的作用得到了进一步表征。BSR0441在巨噬细胞和小鼠感染期间被高度诱导。BSR0441缺失突变体在感染的中晚期脾脏定殖显著减少。在巨噬细胞和小鼠感染期间,BSR0441突变株中BSR0441靶标mRNA基因的表达也发生了改变,这与其细胞内存活能力降低一致。总之,布鲁氏菌编码大量sRNA,它们可能参与布鲁氏菌的应激适应和毒力。对这些调节因子的进一步研究将扩展我们对布鲁氏菌发病机制以及布鲁氏菌与其宿主之间相互作用的理解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22d8/4845025/0468e556c090/srep25123-f1.jpg

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验