Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.
FEMS Microbiol Lett. 2014 May;354(2):119-25. doi: 10.1111/1574-6968.12433. Epub 2014 Apr 16.
Involved in diverse biological processes, bacterial sRNAs are novel regulators of gene expression involved in a wide array of biological processes. To identify sRNAs in Brucella abortus, we performed a genome-wide computational prediction with integrated SIPHT and NAPP results. In total, 129 sRNA candidates were identified, of which 112 were novel sRNA. Twenty novel sRNA candidates were tested by RT-PCR and seven could be verified. The putative targets of these sRNAs were also predicted and verified. This study provides a significant resource for the future study of sRNAs, as well as how sRNAs influence B. abortus physiology and pathogenesis.
参与多种生物过程，细菌 sRNAs 是涉及广泛生物过程的基因表达新的调控因子。为了鉴定布鲁氏菌中的 sRNAs，我们进行了全基因组的计算预测，结合 SIPHT 和 NAPP 的结果。总共鉴定了 129 个 sRNA 候选者，其中 112 个是新的 sRNA。通过 RT-PCR 测试了 20 个新的 sRNA 候选者，其中 7 个可以得到验证。这些 sRNAs 的假定靶标也被预测和验证。本研究为 sRNAs 的未来研究以及 sRNAs 如何影响 B. abortus 的生理和发病机制提供了重要资源。
