Rahman Sajid Ur, Zhu Lei, Cao Li, Zhang Yafei, Chu Xiaoyan, Feng Shibin, Li Yu, Wu Jinjie, Wang Xichun
Department of Clinical Veterinary Medicine, College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, China.
Vet World. 2019;12(4):558-564. doi: 10.14202/vetworld.2019.558-564. Epub 2019 Apr 18.
Brucellosis is one of the most widespread zoonotic diseases globally. Studies indicated the existence of infection in goats in some province of China. Thus this study aimed to estimate the prevalence of brucellosis in goats of Anhui Province, China.
Serum and milk samples obtained from goats in different regions of Anhui province were studied through rose Bengal plate test (RBPT), serum agglutination test (SAT), milk ring test (MRT), and polymerase chain reaction (PCR).
The investigation frequency of brucellosis using RBPT, SAT, MRT, and PCR methods was 3.9% (n=7), 4.45% (n=8), 11.67% (n=7), and 86.67% (n=156), respectively. The prevalence recorded for brucellosis in sex-wise animals as in females 5.55%, 6.67%, 11.67%, and 78.8% through above methods, while in males, it was 2.23% and 2.23% by RBPT and SAT. However, in age-wise animals, the results 6.36%, 7.27%, 11.67%, and 74.5% were perceived positive by RBPT, SAT, MRT, and PCR in adult females, respectively, but young males and females (up to 9 months) were considered free from brucellosis.
These results show that prevalence of brucellosis was relatively higher in females than male's goats and SAT was relatively specific and accurate as compared to RBPT and MRT, but for diagnosis of brucellosis, molecular method (PCR) is recommended.
布鲁氏菌病是全球最广泛传播的人畜共患病之一。研究表明中国某些省份的山羊存在感染情况。因此,本研究旨在评估中国安徽省山羊布鲁氏菌病的流行情况。
通过玫瑰红平板试验(RBPT)、血清凝集试验(SAT)、乳环试验(MRT)和聚合酶链反应(PCR)对从安徽省不同地区采集的山羊血清和乳样进行研究。
采用RBPT、SAT、MRT和PCR方法检测布鲁氏菌病的检出率分别为3.9%(n = 7)、4.45%(n = 8)、11.67%(n = 7)和86.67%(n = 156)。通过上述方法,按性别统计的动物布鲁氏菌病患病率,雌性分别为5.55%、6.67%、11.67%和78.8%,而雄性通过RBPT和SAT检测的患病率为2.23%和2.23%。然而,按年龄统计的动物中,成年雌性通过RBPT、SAT、MRT和PCR检测的阳性结果分别为6.36%、7.27%、11.67%和74.5%,但年轻雄性和雌性(9个月以下)被认为未感染布鲁氏菌病。
这些结果表明,雌性山羊布鲁氏菌病的患病率相对高于雄性,与RBPT和MRT相比,SAT相对特异且准确,但对于布鲁氏菌病的诊断,推荐使用分子方法(PCR)。