Stappers Mark H T, Hagen Ferry, Reimnitz Peter, Mouton Johan W, Meis Jacques F, Gyssens Inge C
Department of Internal Medicine, Radboud University Medical Center, Geert Grooteplein-Zuid 10, 6525 GA, Nijmegen, The Netherlands; Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Weg door Jonkerbos 100, 6532 SZ, Nijmegen, The Netherlands; Hasselt University, Martelarenlaan 42, BE3500, Hasselt, Belgium.
Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Weg door Jonkerbos 100, 6532 SZ, Nijmegen, The Netherlands.
Diagn Microbiol Infect Dis. 2016 Jun;85(2):263-5. doi: 10.1016/j.diagmicrobio.2015.12.017. Epub 2015 Dec 24.
Direct determination by pathogen-specific real-time PCR assay for Bacteroides fragilis was compared to culture in major abscess and diabetic foot infection biopsy samples. Real-time PCR resulted in an increased detection rate of 12% for B. fragilis and could improve the detection of B. fragilis in clinical samples.
将用于脆弱拟杆菌的病原体特异性实时聚合酶链反应（PCR）检测法的直接测定结果与主要脓肿和糖尿病足感染活检样本中的培养结果进行了比较。实时PCR使脆弱拟杆菌的检出率提高了12%，并能改善临床样本中脆弱拟杆菌的检测。
