比较传统 PCR 检测和实时 PCR 检测在临床样本中检测脆弱拟杆菌的效果。
Comparative evaluation of conventional and real-time PCR assays for detecting Bacteroides fragilis in clinical samples.
机构信息
Department of Microbiology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
出版信息
J Clin Microbiol. 2013 May;51(5):1593-5. doi: 10.1128/JCM.00449-13. Epub 2013 Feb 27.
Abstract
A conventional PCR and a real-time PCR for detecting Bacteroides fragilis were evaluated against clinical specimens. Analytical sensitivities were 100 and 40 fg of DNA, respectively. Detection limits were 100 and 10 CFU/ml, respectively. A total of six culture-negative specimens were positive by PCR. Altering the gold standard from "positive culture" to "positive culture or both PCR assays positive" resulted in sensitivities of 91.7% and 100%, respectively, and in specificities of 100% and 98.6%, respectively.
摘要
对检测脆弱拟杆菌的常规 PCR 和实时 PCR 进行了临床标本评估。分析灵敏度分别为 100 fg 和 40 fg DNA。检测限分别为 100 和 10 CFU/ml。总共 6 份培养阴性的标本经 PCR 检测为阳性。将金标准从“阳性培养”改为“阳性培养或两种 PCR 检测均为阳性”，其灵敏度分别为 91.7%和 100%，特异性分别为 100%和 98.6%。
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