Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea, Republic of Korea.
Department of Carbohydrates and Cereals, University of Chemical Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic.
Carbohydr Polym. 2016 Aug 1;146:187-96. doi: 10.1016/j.carbpol.2016.03.043. Epub 2016 Mar 19.
A water-soluble polysaccharide JS-MP-1 was isolated from Korean mulberry fruits Oddi (Morus alba L.). Sugar linkage analysis and NMR data confirmed that it is a rhamnogalacturonan type I (RG I) polymer carrying arabinan and arabinogalactan (AG II) side chains. JS-MP-1 reduced dose-dependently the viability of 3T3-L1 pre-adipocyte cells, significantly stimulated the cleavage of caspases 9 and 3 and poly (ADP-ribose) polymerase (PARP) and decreased the ratio of Bcl-2 to Bax expression level that led to mitochondrial dysfunction and apoptosis in pre-adipocyte cells. The apoptotic death was mediated by stimulation of MAPKs (ERK and p38) signalling pathway. These results suggest that JS-MP-1 is able to reduce the number of fat cells and the mass of adipose tissue via inhibition of pre-adipocyte proliferation and thus JS-MP-1 itself or a crude aqueous Oddi extract containing this polysaccharide can be used as functional ingredient of health-beneficial food supplements for the treatment or prevention of obesity disorders.
一种水溶性多糖 JS-MP-1 从韩国桑椹果实 Oddi（Morus alba L.）中分离得到。糖链分析和 NMR 数据证实，它是一种带有阿拉伯聚糖和阿拉伯半乳聚糖（AG II）侧链的鼠李半乳糖醛酸聚糖 I 型（RG I）聚合物。JS-MP-1 浓度依赖性地降低 3T3-L1 前体脂肪细胞的活力，显著刺激半胱天冬酶 9 和 3 的裂解和多聚（ADP-核糖）聚合酶（PARP），降低 Bcl-2 与 Bax 表达水平的比值，导致前体脂肪细胞中线粒体功能障碍和细胞凋亡。凋亡死亡是通过刺激 MAPKs（ERK 和 p38）信号通路介导的。这些结果表明，JS-MP-1 通过抑制前体脂肪细胞增殖来减少脂肪细胞数量和脂肪组织质量，因此 JS-MP-1 本身或含有该多糖的粗水提 Oddi 提取物可作为治疗或预防肥胖症的功能性食品补充剂的有效成分。
