LBMI, Department of Physics, Lebanese University, Faculty of Sciences II, PO Box 90656, Jdeidet, Lebanon; PRASE, Platform of Research and Analysis in Environmental Sciences, Doctoral School of Sciences and Technologies, Lebanese University, PO Box 5, Hadat, Lebanon; Lyon University, Lyon1 University, IMP@LYON1, UMR CNRS 5223 «Ingénierie des Matériaux Polymères», Villeurbanne, France; Laboratory of Microbiology, Department of Biology, Lebanese University, Faculty of Sciences I, PO Box 5, Hadat, Lebanon.
LBMI, Department of Physics, Lebanese University, Faculty of Sciences II, PO Box 90656, Jdeidet, Lebanon.
Carbohydr Polym. 2016 Aug 1;146:282-91. doi: 10.1016/j.carbpol.2016.02.003. Epub 2016 Feb 17.
Gluconacetobacter genera are valued for bacterial cellulose (BC) and acetic acid production. BC is produced at optimal yields in classical microbiological media that are expensive for a large scale of production. In addition, BC usage for industrial purposes is limited due to low conversion rate into cellulose and to long incubation duration. In this paper, Gluconacetobacter isolated from apple vinegar was kinetically studied to evaluate cellulose production in presence of different carbon sources. Acetic and citric acid effect on Gluconacetobacter metabolism is clarified. It was shown that Gluconacetobacter uses glucose as a primary carbon source for cells growth and products formation. Acetic acid employment as a co-carbon source in Hestrin Schramm medium showed an increase of 17% in BC yield with a moderate decrease in the crystallite size of the resulting polymer.
葡糖醋杆菌属因其生产细菌纤维素 (BC) 和乙酸而受到重视。BC 在经典微生物培养基中以最佳产量生产，但这些培养基的生产成本对于大规模生产来说过高。此外，由于纤维素转化率低和孵育时间长，BC 的工业用途受到限制。在本文中，对从苹果醋中分离出的葡糖醋杆菌进行了动力学研究，以评估不同碳源存在下的纤维素生产情况。阐明了乙酸和柠檬酸对葡糖醋杆菌代谢的影响。结果表明，葡糖醋杆菌将葡萄糖用作细胞生长和产物形成的主要碳源。在 Hestrin Schramm 培养基中使用乙酸作为共碳源，BC 的产量增加了 17%，而所得聚合物的结晶度略有降低。
