Vineetha P G, Tomar S, Saxena V K, Susan C, Sandeep S, Adil K, Mukesh K
a Division of Avian Genetics and Breeding , Utarpradesh , India.
b Division of Veterinary Pathology, Indian Veterinary Research Institute , Utarpradesh , India.
Br Poult Sci. 2016 Aug;57(4):474-82. doi: 10.1080/00071668.2016.1180667. Epub 2016 Jun 29.
A total of 32 Lactobacillus isolates, 8 each from the crop (LGFCP1-LGFCP8), proventriculus (LGFP9-LGFP16), ileum (LGFI17-LGFI24) and caeca (LGFCM25-LGFCM32) were isolated from 25 adult guinea fowl (Pearl variety), 22-28 weeks of age, and characterised morphologically, physiologically, biochemically and by molecular methods. Isolates were screened for their probiotic quality using range of in vitro tests: aggregation test, cell surface hydrophobicity, resistance to bile salts and acidic conditions, enzymatic tests and coaggregation and antagonistic test. Based on in vitro test results and a novel scoring method, the two best isolates were selected and partial 16S rRNA sequencing was done. BLAST (Basic Local Alignment Search Tool) analysis of sequence of isolate LGFCP4 showed 99% genetic identity with Lactobacillus plantarum and LGFP16 with Lactobacillus reuteri. The study shows that these two microbial agents may be suitable as potential probiotic candidates in guinea fowl, as well as in a feed supplement for other poultry species.
从25只22 - 28周龄的成年珍珠鸡中总共分离出32株乳酸杆菌，分别从嗉囊（LGFCP1 - LGFCP8）、腺胃（LGFP9 - LGFP16）、回肠（LGFI17 - LGFI24）和盲肠（LGFCM25 - LGFCM32）各分离出8株，并对其进行形态学、生理学、生物化学及分子方法鉴定。使用一系列体外试验对分离株的益生菌品质进行筛选：聚集试验、细胞表面疏水性、对胆盐和酸性条件的抗性、酶活性试验以及共聚集和拮抗试验。基于体外试验结果和一种新的评分方法，选出了两株最佳分离株并进行了部分16S rRNA测序。分离株LGFCP4序列的BLAST（基本局部比对搜索工具）分析显示与植物乳杆菌有99%的基因同一性，LGFP16与罗伊氏乳杆菌有99%的基因同一性。该研究表明，这两种微生物制剂可能适合作为珍珠鸡以及其他家禽品种饲料添加剂的潜在益生菌候选物。
