通过酶联免疫吸附测定法检测山羊血清中抗支原体F38抗体。
Detection of antibody to mycoplasma F38 in goat sera by an enzyme-linked immunosorbent assay.
作者信息
Wamwayi H M, Wafula J S, Litamoi J K, Nandokha E N
机构信息
Kenya Agricultural Research Institute, Muguga, Kikuyu.
出版信息
Trop Anim Health Prod. 1989 Feb;21(1):43-9. doi: 10.1007/BF02297342.
An indirect enzyme-linked immunosorbent assay (ELISA) was developed to screen goat sera at a single dilution for antibody to mycoplasma F38. Antibody was detected in sera of six convalescent goats following experimental infection. Antibody was also detected in 34 sera three to four weeks after vaccination. No antibody was detected in 164 sera from goats without a history of vaccination or infection with contagious caprine pleuropneumonia. The ELISA was more sensitive than the complement fixation test in detecting antibody in vaccinated goats.
开发了一种间接酶联免疫吸附测定（ELISA），用于在单一稀释度下筛选山羊血清中针对支原体F38的抗体。在实验感染后的6只康复山羊血清中检测到了抗体。在接种疫苗三至四周后的34份血清中也检测到了抗体。在无疫苗接种史或无传染性山羊胸膜肺炎感染史的164份山羊血清中未检测到抗体。在检测接种疫苗山羊的抗体方面，ELISA比补体结合试验更敏感。
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