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用酶联免疫吸附测定法检测牛抗丝状支原体丝状亚种抗体

Detection of antibody against Mycoplasma mycoides subsp mycoides in cattle by an enzyme-linked immunosorbent assay.

作者信息

Onoviran O, Taylor-Robinson D

出版信息

Vet Rec. 1979 Aug 25;105(8):165-6. doi: 10.1136/vr.105.8.165.

Abstract

The results of an enzyme-linked immunosorbent assay (ELISA) For the detection of antibody against Mycoplasma mycoides subsp mycoides are presented. Antibody was detected in the sera of cattle at least 19 months after recovery from an infection and at least 23 months after vaccination. Almost half the sera of some animals in an area of Nigera where contagious bovine pleuropneumonia is enzootic contained antibody. Antibody was rarely detected when the same sera were examined by other established serological tests, emphasising the sensitivity of the ELISA.

摘要

本文展示了用于检测针对丝状支原体丝状亚种抗体的酶联免疫吸附测定(ELISA)结果。在牛从感染中恢复后至少19个月以及接种疫苗后至少23个月的血清中检测到了抗体。在尼日利亚一个传染性牛胸膜肺炎呈地方流行的地区,一些动物的近半数血清含有抗体。当用其他既定血清学检测方法检测相同血清时,很少检测到抗体,这突出了ELISA的敏感性。

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