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[借助沉淀抗体对肌酸激酶同工酶亚基CK-M和CK-B的测定]

[The determination of creatine kinase isoenzyme subunits CK-M and CK-B with the help of precipitating antibodies].

作者信息

Wiedemann G, Böttcher R, Lindenau B, Menzel K

出版信息

Z Med Lab Diagn. 1989;30(2):104-8.

PMID:2711729
Abstract

Isoenzymes of creatine kinase (CK-MM and CK-BB) of samples of tissues from human skeletal muscle and human brain tissues were highly purified. Anti-CK-M and anti-CK-B were prepared by immunization of goats. The obtained antibodies were used in a modified immunoprecipitation method for measuring the creatine kinase subunits of CK-M and CK-B in serum.

摘要

对来自人类骨骼肌和人类脑组织的组织样本中的肌酸激酶同工酶(CK-MM和CK-BB)进行了高度纯化。通过免疫山羊制备了抗CK-M和抗CK-B。所获得的抗体用于一种改良的免疫沉淀法,以测量血清中CK-M和CK-B的肌酸激酶亚基。

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