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[肌酸激酶同工酶的定量免疫化学鉴别。抗肌酸激酶同工酶-MM抗血清的应用]

[Quantitative immunochemical differentiation of creatine kinase isoenzymes. Use of antiserum to isoenzyme MM].

作者信息

Chazov E I, Malakhov V N, Tishchenko V A

出版信息

Biull Eksp Biol Med. 1976 Jun;81(6):659-62.

PMID:986206
Abstract

A possibility of quantitative differentiation of human isoenzymes of creatine kinase by using the antiserum to isoenzyme MM was studied. Rabbit antiserum to human isoenzyme MM proved to specifically inhibit its activity, without altering the isoenzyme BB activity. The use of antiserum to isoenzyme MM as a specific inhibitor makes possible differential determination of the isoenzymes of creatine-kinase or their subunits in the artificial isoenzyme mixtures, and also in the human blood serum and human tissue homogenates.

摘要

研究了使用肌酸激酶同工酶MM抗血清对人肌酸激酶同工酶进行定量区分的可能性。事实证明,兔抗人肌酸激酶同工酶MM血清能特异性抑制其活性,而不改变同工酶BB的活性。使用肌酸激酶同工酶MM抗血清作为特异性抑制剂,能够对人工同工酶混合物、人血清及人体组织匀浆中的肌酸激酶同工酶或其亚基进行鉴别测定。

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