Hou Yanxu, Li Xuebing, Pan Zhenhua, Zu Lingling, Fan Yaguang, You Jiacong, Wang Yuli, Wang Min, Chen Peirui, Shen Wang, Zhou Qinghua
Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China.
Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China;Lung Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhongguo Fei Ai Za Zhi. 2016 Apr 20;19(4):169-76. doi: 10.3779/j.issn.1009-3419.2016.04.11.
Our previous study found that nicotine could induce lung cancer cell epithelial-mesenchymal transition (EMT). The aim of this study is to explore the relationship between nicotine-induced EMT and lung cancer invasion and metastasis.
Real-time PCR and Western blot were used to detect the expression changes of EMT-related markers, E-cadherin and Vimentin, in A549 lung cancer cells treated with nicotine; The transposition of β-catenin protein expression was determined by immunofluorescence; Scratch test and Transwell invasion assay were used to detect the effects of nicotine on lung cancer cell migration and invasion.
Nicotine can significantly down-regulate the expressional level of E-cadherin mRNA and protein of A549 cells in a manner of dose and time-dependent (P<0.01, P<0.01); Nicotine can significantly up-regulate the expressional level of Vimentin mRNA and protein of A549 cells in a manner of dose and time-dependent (P<0.01, P<0.01); Immunofluorescence results showed that β-catenin protein was significantly transfered to nucleus; Scratch test and Transwell assay showed that Nicotine could remarkably increase the migration and invasion potential of lung cancer cells (P<0.01, P<0.01).
Nicotine can induce cancer cells EMT, and promote the invasion and metastasis ability of lung cancer cells.
我们之前的研究发现尼古丁可诱导肺癌细胞上皮-间质转化（EMT）。本研究旨在探讨尼古丁诱导的EMT与肺癌侵袭和转移之间的关系。
采用实时荧光定量PCR和蛋白质印迹法检测尼古丁处理的A549肺癌细胞中EMT相关标志物E-钙黏蛋白和波形蛋白的表达变化；通过免疫荧光法检测β-连环蛋白表达的转位；采用划痕试验和Transwell侵袭试验检测尼古丁对肺癌细胞迁移和侵袭的影响。
尼古丁能以剂量和时间依赖性方式显著下调A549细胞E-钙黏蛋白mRNA蛋白和mRNA的表达水平（P<0.01，P<0.01）；尼古丁能以剂量和时间依赖性方式显著上调A549细胞波形蛋白蛋白和mRNA的表达水平（P<0.01，P<0.01）；免疫荧光结果显示β-连环蛋白蛋白显著转位至细胞核；划痕试验和Transwell试验显示尼古丁可显著提高肺癌细胞的迁移和侵袭能力（P<0.01，P<0.01）。
尼古丁可诱导癌细胞发生EMT，并促进肺癌细胞的侵袭和转移能力。
