do Couto Fabíola Maria Marques, do Nascimento Silene Carneiro, Júnior Silvio Francisco Pereira, da Silva Vanessa Karina Alves, Leal André Ferraz Goiana, Neves Rejane Pereira
Department of Mycology, Federal University of Pernambuco (UFPE), Av. da Engenharia, s/n, Cidade Universitária, Recife, PE CEP 50740-550 Brazil.
Department of Antibiotics, Federal University of Pernambuco (UFPE), Av. da Engenharia, s/n, Cidade Universitária, Recife, PE CEP 50740-550 Brazil.
Springerplus. 2016 Apr 16;5:468. doi: 10.1186/s40064-016-2130-8. eCollection 2016.
This work aimed to evaluate the antifungal activity of piroctone olamine in the treatment of intra-abdominal candidiasis in an experimental model using Swiss mice. The mice (n = 6) were infected by intraperitoneal injection of 0.2 ml of C. albicans (10(7)cells/ml in saline). The animals were observed daily for clinical signs and mortality for 14 days. The treatment with piroctone olamine (0.5 mg/kg) was performed 72 h after infection by intraperitoneal administration. For comparison, a group of animals (n = 6) was treated with amphotericin B (0.5 mg/kg). The mycological diagnosis was made by collecting the liver, spleen and kidneys. Data regarding the fungal growth and mortality were analyzed statistically by Student's t test and analysis of variance (ANOVA), with level of significance set at P < 0.05. The difference in fungal growth scoring between the control group and the treatment groups (piroctone olamine and amphotericin B) was statistically significant (P < 0.05). The difference in fungal growth scoring between the treatment groups (piroctone olamine and amphotericin B) was not statistically significant (P < 0.05).
本研究旨在评估在瑞士小鼠实验模型中，奥麦丁治疗腹腔念珠菌病的抗真菌活性。将小鼠（n = 6）通过腹腔注射0.2 ml白色念珠菌（生理盐水稀释至10(7)个细胞/ml）进行感染。连续14天每日观察动物的临床症状和死亡率。感染72小时后，通过腹腔给药对小鼠进行奥麦丁（0.5 mg/kg）治疗。作为对照，另一组动物（n = 6）用两性霉素B（0.5 mg/kg）治疗。通过采集肝脏、脾脏和肾脏进行真菌学诊断。采用Student's t检验和方差分析（ANOVA）对真菌生长和死亡率数据进行统计学分析，显著性水平设定为P < 0.05。对照组与治疗组（奥麦丁和两性霉素B）之间真菌生长评分的差异具有统计学意义（P < 0.05）。治疗组（奥麦丁和两性霉素B）之间真菌生长评分的差异无统计学意义（P < 0.05）。
