Delemarre F G, Claassen E, Van Rooijen N
Department of Histology, Medical Faculty, Free University, Amsterdam, The Netherlands.
Anat Rec. 1989 Feb;223(2):152-7. doi: 10.1002/ar.1092230206.
Mice were immunized subcutaneously with thymus-independent (TI)-type 1 antigen trinitrophenylated lipopolysaccharide (TNP-LPS), TI-type 2 antigen TNP-Ficoll or thymus-dependent (TD) antigen TNP-keyhole limpet haemocyanin (TNP-KLH) in order to study the primary in situ immune response in popliteal lymph nodes (PLN) and spleen. The spleen responded more rapidly in developing specific antibody-forming cells (AFC) than the lymph nodes did, in spite of the fact that antigens reach the spleen only after passing several lymph node stations. This difference between lymph nodes and spleen in developing AFC was particularly significant with respect to the responses to TI (both type 1 and type 2) antigens. No differences in the distribution of specific AFC in PLN and spleen were observed after immunization with TI and TD antigens. Results are discussed with respect to the relative contributions of lymph nodes and spleen to immune responses to antigens injected subcutaneously.
为了研究腘窝淋巴结（PLN）和脾脏中的原发性原位免疫反应，将小鼠皮下免疫接种非胸腺依赖性（TI）-1型抗原三硝基苯基化脂多糖（TNP-LPS）、TI-2型抗原TNP-菲可（Ficoll）或胸腺依赖性（TD）抗原三硝基苯基化钥孔戚血蓝蛋白（TNP-KLH）。尽管抗原只有在经过几个淋巴结站后才到达脾脏，但脾脏在产生特异性抗体形成细胞（AFC）方面的反应比淋巴结更快。在产生AFC方面，淋巴结和脾脏之间的这种差异在对TI（1型和2型）抗原的反应中尤为显著。用TI和TD抗原免疫后，未观察到PLN和脾脏中特异性AFC分布的差异。讨论了关于淋巴结和脾脏对皮下注射抗原的免疫反应的相对贡献。
