Van Den Berg T K, Hasbold J, Renardel De Lavalette C, Döpp E A, Dijkstra C D, Klaus G G
Department of Cell Biology and Immunology, Vrije University, Amsterdam, Netherlands.
Immunology. 1996 Jun;88(2):294-300. doi: 10.1111/j.1365-2567.1996.tb00018.x.
In this study we describe the tissue distribution of mouse CD40 using two monoclonal antibodies (mAb) against different epitopes of the molecule. In lymphoid tissues CD40 was expressed by B lymphocytes. Most B cells in typical B-cell compartments were CD40-positive, including germinal centre B cells. Interestingly, the two CD40 epitopes were differentially distributed on subpopulations of dendritic cells and epithelial cells. The 3/23 mAb, but not 3/3, recognized interdigitating dendritic cells (IDC) in lymph nodes, spleen and thymus. Langerhans cells were CD40 negative. In contrast, epithelial cells in the thymus and some other tissues (e.g. skin) were stained with the 3/3 mAb, but not with the 3/23 mAb. The expression of CD40 on dendritic cells and epithelial cells is in agreement with earlier findings in humans. Our data also demonstrate that different epitopes of CD40 are differentially expressed on dendritic cells and epithelial cells. This suggests the existence of different forms of CD40, that are expressed in a cell-type-specific fashion.
在本研究中，我们使用针对该分子不同表位的两种单克隆抗体（mAb）描述了小鼠CD40的组织分布。在淋巴组织中，CD40由B淋巴细胞表达。典型B细胞区室中的大多数B细胞为CD40阳性，包括生发中心B细胞。有趣的是，这两种CD40表位在树突状细胞和上皮细胞亚群上的分布存在差异。3/23 mAb能识别淋巴结、脾脏和胸腺中的交错突树突状细胞（IDC），而3/3 mAb不能。朗格汉斯细胞CD40阴性。相反，胸腺和其他一些组织（如皮肤）中的上皮细胞能用3/3 mAb染色，但不能用3/23 mAb染色。树突状细胞和上皮细胞上CD40的表达与人类早期研究结果一致。我们的数据还表明，CD40的不同表位在树突状细胞和上皮细胞上的表达存在差异。这表明存在不同形式的CD40，它们以细胞类型特异性方式表达。
