Rui H, Purvis K, Gordeladze J O
Institute of Pathology, National Hospital, Oslo, Norway.
Andrologia. 1989 Mar-Apr;21(2):131-5.
The present report is an extension of a previous study of the accessory sex gland function in normal young (20-25 years) and middle-aged (50-55 years) men. In the same individuals, the basal and in vitro stimulated activities of the sperm adenylyl cyclase were determined. A high degree of individual variation existed in both basal and Gpp(NH)p-, forskolin- and acetate-stimulated sperm adenylyl cyclase, and no significant difference could be demonstrated between the means of the age groups. Furthermore, no significant correlation existed between the concentrations of the glandular secretory products (prostatic acid phosphatase, citric acid, zinc, spermine, spermidine, putrescine, fructose and prostaglandin E) in the ejaculate and the activity of the sperm adenylyl cyclase. Furthermore, the number of morphologically abnormal sperm cells in the ejaculate did not influence the activity of the enzyme. No inverse correlation existed between the magnitude of basal activity and the relative response to Gpp(NH)p, forskolin or acetate suggesting that the variation in the enzyme could not be explained by its partial desensitization by seminal plasma constituents during the liquefaction period. A lack of correlation between the response of the enzyme to Gpp(NH) p and forskolin on the one hand and acetate on the other, implied that these drugs activate the enzyme through different mechanisms.
本报告是之前一项关于正常年轻（20 - 25岁）和中年（50 - 55岁）男性附属性腺功能研究的扩展。对同一批个体，测定了精子腺苷酸环化酶的基础活性以及体外刺激后的活性。精子腺苷酸环化酶的基础活性以及经Gpp(NH)p、福斯高林和乙酸盐刺激后的活性均存在高度个体差异，且各年龄组均值之间无显著差异。此外，射精液中腺分泌产物（前列腺酸性磷酸酶、柠檬酸、锌、精胺、亚精胺、腐胺、果糖和前列腺素E）的浓度与精子腺苷酸环化酶的活性之间无显著相关性。而且，射精液中形态异常精子细胞的数量不影响该酶的活性。基础活性的大小与对Gpp(NH)p、福斯高林或乙酸盐的相对反应之间不存在负相关，这表明该酶的变化不能用液化期精浆成分使其部分脱敏来解释。一方面，该酶对Gpp(NH)p和福斯高林的反应与另一方面对乙酸盐的反应之间缺乏相关性，这意味着这些药物通过不同机制激活该酶。
