Magnus O, Purvis K, Rui H, Clausen O P, Abyholm T, Gordeladze J O
Arch Androl. 1986;17(3):165-72. doi: 10.3109/01485018608990192.
Studies of the adenylyl cyclase in spermatozoa from 10 asthenozoospermic and 10 normal men revealed major differences with regard to the activation of the enzyme by various drugs and metal ions. While the response to acetate ions was normal, activation of the enzyme by forskolin and the GTP analogue [Gpp(NH)p] was uniformly impaired. In addition, in 4 of the patients the ratios of the Mn2+- and Mg2+-dependent activities of the enzyme were significantly higher than normal, suggesting a delay in the transformation of the enzymes catalytic unit from the soluble to the membrane-bound form. No differences could be recorded in a variety of seminal plasma components including prostatic acid phosphatase, citric acid, zinc, putrescine, spermidine, spermine, fructose, prostaglandin E, and testosterone. Furthermore, the DNA fluorescence patterns of the spermatozoa measured by flow cytometry were within the range of control variations, suggesting that chromatin condensation, and thus nuclear maturation, proceeded normally. The study suggests that a lesion in the adenylyl cyclase system of the germ cells may explain at least some of the motility disturbances seen in infertile males.
对10名弱精子症男性和10名正常男性精子中的腺苷酸环化酶进行的研究显示，在各种药物和金属离子对该酶的激活方面存在重大差异。虽然对醋酸根离子的反应正常，但福斯高林和GTP类似物[Gpp(NH)p]对该酶的激活均受到损害。此外，4名患者中该酶的锰离子依赖性活性与镁离子依赖性活性之比显著高于正常水平，这表明酶催化单位从可溶性形式向膜结合形式的转化存在延迟。在包括前列腺酸性磷酸酶、柠檬酸、锌、腐胺、亚精胺、精胺、果糖、前列腺素E和睾酮在内的多种精浆成分中未记录到差异。此外，通过流式细胞术测量的精子DNA荧光模式在对照变化范围内，这表明染色质浓缩以及核成熟过程正常进行。该研究表明，生殖细胞腺苷酸环化酶系统的损伤可能至少解释了不育男性中观察到的一些运动障碍。
