Yoon Jaewoo, Adachi Kyoko, Kasai Hiroaki
College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 42601, Republic of Korea.
Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate, 026-0001, Japan.
Arch Microbiol. 2016 Sep;198(7):663-70. doi: 10.1007/s00203-016-1227-3. Epub 2016 Apr 28.
A Gram-stain-negative, strictly aerobic, pale-yellow pigmented, rod-shaped, chemoheterotrophic bacterium, designated A6F-11(T), was isolated from a marine sponge collected in Japan. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the novel marine strain was affiliated with the family Flavobacteriaceae of the phylum Bacteroidetes and that it shared the highest (92.9 %) sequence similarity with Arenibacter palladensis LMG 21972(T). The strain could be differentiated phenotypically from related members of the family Flavobacteriaceae. The major fatty acids of strain A6F-11(T) were iso-C15:1 G, iso-C15:0, C16:1 ω6c and/or C16:1 ω7c and iso-C17:0 3-OH. The polar lipid profile consisted of phosphatidylglycerol, two unidentified aminolipids and two unidentified lipids. The DNA G+C content was 34.7 mol%, and the major respiratory quinone was menaquinone 6 (MK-6). From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel taxon in the family Flavobacteriaceae, for which the name Spongiimicrobium salis gen. nov., sp. nov. is proposed. The type strain of S. salis gen. nov., sp. nov. is A6F-11(T) (= KCTC 42753(T) = NBRC 111401(T)).
从日本采集的海洋海绵中分离出一株革兰氏染色阴性、严格需氧、淡黄色色素、杆状、化能异养细菌，命名为A6F-11(T)。基于16S rRNA基因序列的系统发育分析表明，该新型海洋菌株隶属于拟杆菌门黄杆菌科，与苍白嗜盐杆菌LMG 21972(T)的序列相似性最高（92.9%）。该菌株在表型上可与黄杆菌科的相关成员区分开来。菌株A6F-11(T)的主要脂肪酸为异-C15:1 G、异-C15:0、C16:1 ω6c和/或C16:1 ω7c以及异-C17:0 3-OH。极性脂质谱由磷脂酰甘油、两种未鉴定的氨基脂质和两种未鉴定的脂质组成。DNA G+C含量为34.7 mol%，主要呼吸醌为甲基萘醌6（MK-6）。基于独特的系统发育位置以及基因型和表型特征的组合，该菌株被认为代表黄杆菌科中的一个新分类单元，为此提出新属名海绵微菌属（Spongiimicrobium）、新种名盐海绵微菌（Spongiimicrobium salis）。盐海绵微菌属新种的模式菌株为A6F-11(T)（=KCTC 42753(T)=NBRC 111401(T)）。
