Yoon Jaewoo, Adachi Kyoko, Kasai Hiroaki
College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-gu, Daegu, 42601, Republic of Korea.
Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate, 026-0001, Japan.
Curr Microbiol. 2016 Jul;73(1):15-21. doi: 10.1007/s00284-016-1022-z. Epub 2016 Mar 10.
A Gram stain-negative, strictly aerobic, brown-pigmented, non-motile, rod-shaped, chemoheterotrophic bacterial strain-designated A6F-119(T) was isolated from a marine sponge (Rhabdastrella sp.). Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the new strain represented a member of the family Flavobacteriaceae of the phylum Bacteroidetes and that it showed highest sequence similarity (93 %) to Tenacibaculum maritimum NBRC 15946(T). The strain could be differentiated phenotypically from the recognized members of the family Flavobacteriaceae. The DNA G + C content of strain A6F-119(T) was determined to be 30.8 mol%; MK-6 was identified as the major menaquinone; and the presence of iso-C15:0, iso-C17:0 3-OH, and C16:1 ω7c and/or C16:1 ω6c as the major (>10 %) cellular fatty acids. A polar lipid profile was present consisting of phosphatidylethanolamine, an unidentified aminolipid, and three unidentified lipids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel genus for which the name Spongiiferula fulva gen. nov., sp. nov. is proposed. The type strain of S. fulva is A6F-119(T) (= KCTC 42752(T) = NBRC 111402(T)).
从一种海洋海绵（Rhabdastrella属）中分离出一株革兰氏阴性、严格需氧、产棕色色素、不运动、杆状、化能异养细菌菌株，命名为A6F-119(T)。基于16S rRNA基因序列的系统发育分析表明，该新菌株是拟杆菌门黄杆菌科的一个成员，与海生黄杆菌NBRC 15946(T)的序列相似性最高（93%）。该菌株在表型上可与黄杆菌科已确认的成员区分开来。菌株A6F-119(T)的DNA G + C含量测定为30.8 mol%；鉴定出MK-6为主要甲基萘醌；主要（>10%）细胞脂肪酸为异-C15:0、异-C17:0 3-OH以及C16:1 ω7c和/或C16:1 ω6c。存在由磷脂酰乙醇胺、一种未鉴定的氨基脂质和三种未鉴定的脂质组成的极性脂质谱。基于独特的系统发育位置以及基因型和表型特征的组合，该菌株被认为代表一个新属，为此提出新属名Spongiiferula fulva gen. nov., sp. nov.。S. fulva的模式菌株是A6F-119(T)（= KCTC 42752(T) = NBRC 111402(T)）。
