Autio I, Malo-Ranta U, Kallioniemi O P, Nikkari T
Department of Biomedical Sciences, University of Tampere, Finland.
Artery. 1989;16(2):72-83.
Interactions of vascular endothelial cells (ECs) and smooth muscle cells (SMCs) were studied by testing the ability of cultured bovine aortic ECs to secrete factors influencing the migration of cultured aortic SMCs from the same species. Migration of SMCs was examined in blind-well chambers using gelatin-coated polycarbonate filters. Conditioned culture medium obtained by incubating confluent monolayers of ECs in serum-free RPMI-1640 medium for 48 hours caused a 2.4-fold increase in the migration of SMCs as compared with nonconditioned medium (p less than 0.001). The effect was dependent on the length of conditioning with the ECs and was chemotactic in nature as judged on the basis of checkerboard analysis. Preliminary characterization of the migration stimulating activity indicates that it is sensitive to trypsin, nondialyzable, and stable at 56 degrees C for 30 min. The activity was abolished by heating to 100 degrees C for 20 min but was not significantly inhibited by protamine sulphate, which suggests that most of the activity was not due to platelet-derived growth factor (PDGF)-like proteins. Our results thus show that ECs secrete polypeptide(s) chemotactic for vascular SMCs. Such interactions between ECs and SMCs in vivo might contribute to the migration of medial SMCs into the intima during atherogenesis.
通过检测培养的牛主动脉内皮细胞（ECs）分泌影响同物种培养的主动脉平滑肌细胞（SMCs）迁移的因子的能力，研究了血管内皮细胞与平滑肌细胞的相互作用。使用明胶包被的聚碳酸酯滤膜在盲孔小室中检测平滑肌细胞的迁移。将汇合的内皮细胞单层在无血清的RPMI-1640培养基中孵育48小时获得的条件培养基，与非条件培养基相比，使平滑肌细胞的迁移增加了2.4倍（p小于0.001）。该效应取决于与内皮细胞的孵育时间，并且根据棋盘分析判断其本质上是趋化性的。对迁移刺激活性的初步表征表明，它对胰蛋白酶敏感，不可透析，并且在56℃下30分钟稳定。加热至100℃20分钟可消除该活性，但硫酸鱼精蛋白对其无明显抑制作用，这表明大部分活性并非源于血小板衍生生长因子（PDGF）样蛋白。因此，我们的结果表明内皮细胞分泌对血管平滑肌细胞具有趋化作用的多肽。体内内皮细胞与平滑肌细胞之间的这种相互作用可能有助于动脉粥样硬化形成过程中中膜平滑肌细胞向内膜的迁移。
