Luo Fei, Liu Tong, Wang Jianan, Li Jian, Ma Pengde, Ding Hao, Feng Guowei, Lin Dong, Xu Yong, Yang Kuo
Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin, China.
Department of Urology, Tianjin Union Medical Center, Tianjin, China.
Oncotarget. 2016 May 24;7(21):30924-34. doi: 10.18632/oncotarget.9045.
The tumor microenvironment is comprised of diverse stromal cells that contribute towards tumor progression. As a result, there has been a growing interest in the role of bone marrow derived cells (BMDCs) in cancer progression. However, the role of BMDCs in prostate cancer (PCa) progression still remains unclear. In this study, we established GFP bone marrow transplanted TRAMP and MUN-induced prostate cancer models, in order to investigate the role of BMDCs in prostate cancer progression. By tracing GFP positive cells, we observed that BMDCS were recruited into mouse prostate tissues during tumorigenesis. GFP+/Sca-1+/CD45- BMDCs were significantly increased in the MNU-induced PCa group, as compared to the citrated-treated control group (2.67 ± 0.25% vs 0.67 ± 0.31%, p = 0.006). However, there were no significant differences found in GFP+/Sca-1+/CD45+ cell populations between the two groups (0.27 ± 0.15% vs 0.10 ± 0.10%, p = 0.334). Moreover, co-grafting of bone marrow mesenchymal stem cells (BMMSCs) and RM1 cells were found to promote RM1 tumor growth in vivo, and cell fusion was observed in RM-1+BMMSCs xenografts. Therefore, the data suggests that BMDCs can be recruited to the prostate during carcinogenesis, and that BMMSCs may promote the growth of PCa.
肿瘤微环境由多种促进肿瘤进展的基质细胞组成。因此，人们对骨髓来源细胞（BMDCs）在癌症进展中的作用越来越感兴趣。然而，BMDCs在前列腺癌（PCa）进展中的作用仍不清楚。在本研究中，我们建立了绿色荧光蛋白（GFP）骨髓移植的TRAMP和MNU诱导的前列腺癌模型，以研究BMDCs在前列腺癌进展中的作用。通过追踪GFP阳性细胞，我们观察到在肿瘤发生过程中BMDCS被募集到小鼠前列腺组织中。与柠檬酸盐处理的对照组相比，MNU诱导的PCa组中GFP+/Sca-1+/CD45- BMDCs显著增加（2.67±0.25%对0.67±0.31%，p = 0.006）。然而，两组之间GFP+/Sca-1+/CD45+细胞群没有显著差异（0.27±0.15%对0.10±0.10%，p = 0.334）。此外，发现骨髓间充质干细胞（BMMSCs）与RM1细胞共移植可促进RM1肿瘤在体内生长，并且在RM-1+BMMSCs异种移植中观察到细胞融合。因此，数据表明BMDCs在致癌过程中可被募集到前列腺，并且BMMSCs可能促进PCa的生长。
