Wang Junrong, Zhang Nina, Qu Haijiang, You Guangxian, Yuan Junhui, Chen Caie, Li Wenyi, Pan Feng
Department of Laboratory Medicine, Wenling Maternal and Child Health Care Hospital, Wenling 317500, Zhejiang Province, China.
Department of Oncology, The Second People's Hospital of Wenling City (Cancer Hospital in Taizhou, Shanghai Tumor Hospital in Taizhou Branch), Wenling 317502, Zhejiang Province, China.
Biosci Rep. 2016 Jun 3;36(3). doi: 10.1042/BSR20160072. Print 2016 Jul.
To investigate the effects of signal transducer and activator of transcription 3 (STAT3) combined with cisplatin (CDDP) on the growth of human Wilms tumour (WT) SK-NEP-1 cell subcutaneous xenografts in nude mice and the possible mechanisms. Human WT SK-NEP-1 cells were subcutaneously transplanted to establish the BALB/c nude mice xenograft model. Mice were randomly divided into five groups: blank control group, adenovirus control group (NC group), STAT3 group, CDDP group and STAT3 plus CDDP group (combination group). Tumour volume and tumour weight were observed during the therapeutic process. The expression levels of STAT3, glucose regulatory protein 78 (GRP78) and BCL2-associated X protein (BAX) were evaluated by immunohistochemical analysis. Compared with the STAT3 group or CDDP group, the tumour weight and volume was significantly reduced in the combination group (P<0.05). No statistical significance was found in NC group compared with the blank control group (P > 0.05). Immunohistochemical analysis showed that STAT3, GRP78 and BAX protein levels in the combination group were significantly higher than those in STAT3 group and CDDP group (P<0.05). Exogenous STAT3 and CDDP may synergistically inhibit the xenograft tumour growth through up-regulation of BAX protein via GRP78.
探讨信号转导与转录激活因子3（STAT3）联合顺铂（CDDP）对人肾母细胞瘤（WT）SK-NEP-1细胞皮下异种移植瘤在裸鼠体内生长的影响及可能机制。将人WT SK-NEP-1细胞皮下移植建立BALB/c裸鼠异种移植瘤模型。小鼠随机分为五组：空白对照组、腺病毒对照组（NC组）、STAT3组、CDDP组和STAT3加CDDP组（联合组）。在治疗过程中观察肿瘤体积和肿瘤重量。通过免疫组织化学分析评估STAT3、葡萄糖调节蛋白78（GRP78）和BCL2相关X蛋白（BAX）的表达水平。与STAT3组或CDDP组相比，联合组的肿瘤重量和体积显著降低（P<0.05）。NC组与空白对照组相比无统计学意义（P>0.05）。免疫组织化学分析显示，联合组中STAT3、GRP78和BAX蛋白水平显著高于STAT3组和CDDP组（P<0.05）。外源性STAT3和CDDP可能通过GRP78上调BAX蛋白协同抑制异种移植瘤生长。
