Xia Zhi-Jie, Wu Hong-Zhen, Cui Chun-Xiao, Chen Qi, Zhao Guo-Yan, Wang Hai-Xia, Dai Mei-Xue
College of Life Science, Shandong Normal University, Jinan 250014, PR China.
Medical College, Shandong Xiehe University, Jinan 250107, PR China.
Int J Syst Evol Microbiol. 2016 Aug;66(8):2952-2958. doi: 10.1099/ijsem.0.001126. Epub 2016 Apr 29.
A moderately halophilic, Gram-stain-negative, non-endospore-forming endophytic bacterium designated strain ST307T was isolated from the euhalophyte Suaeda salsa in Dongying, China. Strain ST307T was aerobic, rod-shaped, motile and orange-yellow-pigmented. The organism grew at NaCl concentrations of 0.6-20 % (w/v) (optimum 5-6 %, w/v), at temperatures of 5-45 °C (optimum 35 °C) and at pH 5-9 (optimum pH 7-8). It accumulated poly-β-hydroxybutyric acid and produced exopolysaccharides. The major fatty acids were C18 : 1ω7c/C18 : 1ω6c, C16 : 0 and C16 : 1ω7c/C16 : 1ω6c. The predominant lipoquinone was ubiquinone Q-9. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, a glycoaminolipid and a phosphoglycoaminolipid. The DNA G+C content was 60.5 mol%. Phylogenetic analyses of 16S rRNA gene sequences and concatenated atpA, rpoD and secA gene sequences revealed that the strain represents a member of the genus Larsenimonas. The closest related type strain was Larsenimonas salina M1-18T. Mean DNA-DNA relatedness values between strain ST307T and the related species L. salina M1-18T, Chromohalobacter beijerinckii DSM 7218T, C. canadensis DSM 6769T, C. israelensis DSM 6768T, C. marismortui CGMCC 1.2321T, C. nigrandesensis DSM 14323T, C. salexigens DSM 3043T and C. sarecensis DSM 15547T were 15±2-45±1 %. On the basis of phenotypic, chemotaxonomic and molecular features, strain ST307T clearly represents a novel species of the genus Larsenimonas. The name Larsenimonassuaedae sp. nov. is proposed, with ST307T (=CGMCC 1.8902T=DSM 22428T) as the type strain.
从中国东营的真盐生植物盐地碱蓬中分离出一株中度嗜盐、革兰氏染色阴性、不形成芽孢的内生细菌，命名为ST307T菌株。ST307T菌株为需氧菌，杆状，具运动性，产橙黄色色素。该菌在NaCl浓度为0.6 - 20%（w/v）（最适浓度为5 - 6%，w/v）、温度为5 - 45℃（最适温度为35℃）和pH值为5 - 9（最适pH值为7 - 8）的条件下生长。它能积累聚-β-羟基丁酸并产生胞外多糖。主要脂肪酸为C18:1ω7c/C18:1ω6c、C16:0和C16:1ω7c/C16:1ω6c。主要的脂醌为泛醌Q-9。极性脂质由磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、一种糖胺脂和一种磷酸糖胺脂组成。DNA的G+C含量为60.5 mol%。对16S rRNA基因序列以及串联的atpA、rpoD和secA基因序列进行系统发育分析表明，该菌株是拉森氏单胞菌属的一个成员。最接近的相关模式菌株是盐拉森氏单胞菌M1-18T。ST307T菌株与相关物种盐拉森氏单胞菌M1-18T、拜耶林克嗜盐杆菌DSM 7218T、加拿大嗜盐杆菌DSM 6769T、以色列嗜盐杆菌DSM 6768T、死海嗜盐杆菌CGMCC 1.2321T、黑色嗜盐杆菌DSM 143233、盐生嗜盐杆菌DSM 3043T和萨雷西嗜盐杆菌DSM 15547T之间的平均DNA-DNA相关性值为15±2 - 45±1%。基于表型、化学分类和分子特征，ST307T菌株显然代表了拉森氏单胞菌属的一个新物种。提议将其命名为盐地碱蓬拉森氏单胞菌（Larsenimonassuaedae sp. nov.），以ST307T（=CGMCC 1.8902T=DSM 22428T）作为模式菌株。
