Zhang Lei, Pan Yuanyuan, Wang Kaibiao, Zhang Xiaoxia, Zhang Cheng, Zhang Shuang, Fu Xiaowei, Jiang Juquan
Department of Microbiology and Biotechnology, College of Life Sciences, Northeast Agricultural University, Harbin, 150030, PR China.
Agricultural Cultural Collection of China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, PR China.
Int J Syst Evol Microbiol. 2015 Mar;65(Pt 3):1022-1030. doi: 10.1099/ijs.0.000057. Epub 2015 Jan 8.
Strain NEAU-ST5-21(T) was isolated from saline and alkaline soils in Zhaodong City, Heilongjiang Province, China. It was aerobic, Gram-stain-negative, rod-shaped and motile with a polar flagellum. It produced yellow-orange colonies with a smooth surface, and grew in the presence of 0-5 % (w/v) NaCl (optimum 0 %, w/v), at temperatures of 20-40 °C (optimum 28 °C) and at pH 7-11 (optimum pH 7). Phylogenetic analyses based on the separate 16S rRNA gene sequences and concatenated 16S rRNA, gyrB and rpoD gene sequences indicated that strain NEAU-ST5-21(T) belongs to the genus Pseudomonas in the class Gammaproteobacteria. The most closely related species is Pseudomonas xanthomarina, whose type strain (KMM 1447(T)) showed gene sequence similarities of 99.0 % for 16S rRNA, 81.8 % for gyrB and 85.0 % for rpoD with strain NEAU-ST5-21(T). DNA-DNA hybridization values between strain NEAU-ST5-21(T) and P. xanthomarina DSM 18231(T), Pseudomonas kunmingensis CGMCC 1.12273(T), Pseudomonas stutzeri DSM 5190(T), Pseudomonas oleovorans subsp. lubricantis DSM 21016(T), Pseudomomas chengduensis CGMCC 2318(T), Pseudomonas alcaliphila DSM 17744(T) and Pseudomonas toyotomiensis DSM 26169(T) were 52±0 % to 25±2 %. The DNA G+C content of strain NEAU-ST5-21(T) was 65 mol%. The major fatty acids (>10 %) were C18 : 1ω7c and/or C18 : 1ω6c, C16 : 1ω7c and/or C16 : 1ω6c and C16 : 0, the predominant respiratory quinone was ubiquinone 9, and polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid, phosphatidylglycerol, one unknown aminolipid, one unknown lipid and a glycolipid. The proposed name is Pseudomonas zhaodongensis sp. nov., NEAU-ST5-21(T) ( = ACCC 06362(T) = DSM 27559(T)) being the type strain.
菌株NEAU-ST5-21(T)从中国黑龙江省肇东市的盐碱土中分离得到。它是需氧菌，革兰氏染色阴性，杆状，具极生鞭毛，能运动。它产生表面光滑的黄橙色菌落，能在0-5%（w/v）NaCl（最适0%，w/v）、20-40℃（最适28℃）和pH 7-11（最适pH 7）条件下生长。基于单独的16S rRNA基因序列以及串联的16S rRNA、gyrB和rpoD基因序列的系统发育分析表明，菌株NEAU-ST5-21(T)属于γ-变形菌纲假单胞菌属。与其关系最密切的物种是黄斑假单胞菌，其模式菌株（KMM 1447(T)）与菌株NEAU-ST5-21(T)的16S rRNA基因序列相似性为99.0%，gyrB为81.8%，rpoD为85.0%。菌株NEAU-ST5-21(T)与黄斑假单胞菌DSM 18231(T)、昆明假单胞菌CGMCC 1.12273(T)、施氏假单胞菌DSM 5190(T)、食油假单胞菌滑润亚种DSM 21016(T)、成都假单胞菌CGMCC 2318(T)、嗜碱假单胞菌DSM 17744(T)和丰臣假单胞菌DSM 26169(T)之间的DNA-DNA杂交值为52±0%至25±2%。菌株NEAU-ST5-21(T)的DNA G+C含量为65 mol%。主要脂肪酸（>10%）为C18 : 1ω7c和/或C18 : 1ω6c、C16 : 1ω7c和/或C16 : 1ω6c以及C16 : 0，主要呼吸醌为泛醌9，极性脂质由双磷脂酰甘油、磷脂酰乙醇胺、一种未知磷脂、磷脂酰甘油、一种未知氨基脂质、一种未知脂质和一种糖脂组成。提议将其命名为肇东假单胞菌新种，NEAU-ST5-21(T)（=ACCC 06362(T)=DSM 27559(T)）为模式菌株。
