Zhang Wenbo, Huo Yanhong, Wang Xinling, Jia Yingmin, Su Li, Wang Caixia, Li Ying, Yang Yonghong, Liu Yuanyuan
Department of Nephrology, The General Hospital of Beijing Military District, Beijing, 100700, People's Republic of China.
Department of Information, The General Hospital of Beijing Military District, Beijing, 100700, People's Republic of China.
Heart Vessels. 2016 Nov;31(11):1874-1881. doi: 10.1007/s00380-016-0834-x. Epub 2016 Apr 29.
The differentiation rate of adipose-derived mesenchymal stem cells (Ad-MSCs) into endothelial cells is always lower under normal condition, which limits further clinical application of Ad-MSCs for angiogenesis regenerative medicine and needs to be enhanced. In the present study, the tissue-specific-derived decellularized ovine arteries matrix (DCS) was used as scaffold to investigate the pro-endothelial differentiation ability of decellularized ovine arteries matrix as well as the underlying mechanisms. The prepared decellularized ovine arteries matrix by the combination of enzymatic and chemical decellularization approaches preserved macroscopic 3D architecture, native composition and ultrastructure of natural ovine arteries. The RT-PCR, histopathological and immunofluorescence assay results suggested that DCS could increase the proliferation ability of MSC. What's more, the DCS could also induce the endothelial differentiation of MSC, which was further enhanced by adding VEGF. Our results showed that natural 3D matrix from decellularized ovine arteries could induce the endothelial differentiation of AD-MSCs alone or with the combination of VEGF. Our results indicated that the decellularized ovine arteries matrix would serve as an efficient culture system for promoting endothelial differentiation of Ad-MSCs.
在正常条件下，脂肪来源的间充质干细胞（Ad-MSCs）向内皮细胞的分化率一直较低，这限制了Ad-MSCs在血管生成再生医学中的进一步临床应用，因此需要提高其分化率。在本研究中，将组织特异性来源的去细胞化绵羊动脉基质（DCS）用作支架，以研究去细胞化绵羊动脉基质促进内皮细胞分化的能力及其潜在机制。通过酶解和化学去细胞化方法相结合制备的去细胞化绵羊动脉基质保留了天然绵羊动脉的宏观三维结构、天然成分和超微结构。逆转录聚合酶链反应（RT-PCR）、组织病理学和免疫荧光检测结果表明，DCS可提高间充质干细胞的增殖能力。此外，DCS还可诱导间充质干细胞向内皮细胞分化，添加血管内皮生长因子（VEGF）可进一步增强这种分化。我们的结果表明，去细胞化绵羊动脉的天然三维基质可单独或与VEGF联合诱导Ad-MSCs向内皮细胞分化。我们的结果表明，去细胞化绵羊动脉基质将作为一种有效的培养系统，用于促进Ad-MSCs向内皮细胞分化。
