Bilal Muhammad, Asgher Muhammad, Iqbal Munawar, Hu Hongbo, Zhang Xuehong
State Key Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Industrial Biotechnology Laboratory, Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan.
Int J Biol Macromol. 2016 Aug;89:181-9. doi: 10.1016/j.ijbiomac.2016.04.075. Epub 2016 Apr 26.
Textile industry has led to severe environmental pollution and is posing a serious threat to the ecosystems. Immobilized biocatalysts have gained importance as potential bio-remediating agent. Manganese peroxidase (MnP) was immobilized onto glutaraldehyde activated chitosan beads by crosslinking and employed for the degradation and detoxification of dyes in textile effluents. The efficiency of chitosan-immobilized MnP (CI-MnP) was evaluated on the basis of decolorization, water quality improvement and toxicity reduction. Maximum color removal of 97.31% was recorded and up to 82.40%, 78.30% and 91.7% reductions in COD, TOC, and BOD were achieved, respectively. The cytotoxicity of bio-treated effluents reduced significantly and 38.46%, 43.47% and 41.83% Allium cepa root length, root count and mitotic index were increased, respectively, whereas brine shrimp nauplii death reduced up to 63.64%. Mutagenicity (Ames test) reduced up to 73.44% and 75.43% for TA98 and TA100 strains, respectively. The CI-MnP retained 60% activity after 10 repeated decolorization batches. The CI-MnP showed excellent efficiency for the bioremediation of textile effluents and can be used for the remediation of toxic agents in wastewater. The monitoring of processed wastewater using bioassays is suggested to evaluate bio-efficiency of treatment method for safe disposal of effluents into water bodies.
纺织工业已导致严重的环境污染，并对生态系统构成严重威胁。固定化生物催化剂作为潜在的生物修复剂已变得越来越重要。通过交联将锰过氧化物酶（MnP）固定在戊二醛活化的壳聚糖珠上，并用于纺织废水中染料的降解和解毒。基于脱色、水质改善和毒性降低来评估壳聚糖固定化MnP（CI-MnP）的效率。记录到的最大脱色率为97.31%，化学需氧量（COD）、总有机碳（TOC）和生化需氧量（BOD）分别降低了82.40%、78.30%和91.7%。生物处理后废水的细胞毒性显著降低，洋葱根尖长度、根数量和有丝分裂指数分别增加了38.46%、43.47%和41.83%，而卤虫无节幼体死亡率降低了63.64%。对于TA98和TA100菌株，致突变性（艾姆斯试验）分别降低了73.44%和75.43%。在10个重复脱色批次后，CI-MnP保留了60%的活性。CI-MnP对纺织废水的生物修复显示出优异的效率，可用于废水中有毒物质的修复。建议使用生物测定法监测处理后的废水，以评估处理方法的生物效率，以便将废水安全排放到水体中。
