Smirniotis V E, Labrou A T, Tsiftses D D
Athens University, Medical School, Second Department of Surgery, Areteion Hospital, Greece.
Ann Vasc Surg. 1989 Jan;3(1):8-10. doi: 10.1016/S0890-5096(06)62377-7.
Changes in the serum of CPK-BB isoenzyme were investigated in dogs with intestinal ischemia in order to determine their use as an enzymatic diagnostic test for bowel infarction. The study consisted of 12 mongrel dogs. Group I (n = 6) was assigned as a control and was subjected to lumen obstruction of a jejunal segment 50-60 cm in length. Group II (n = 6) had an intestinal infarction created by ligation of the arteries of a jejunal segment 50-60 cm in length. A catheter was placed into the right atrium through the left jugular vein for blood sampling and monitoring. Blood samples were withdrawn every three hours for biochemical assays. CPK-BB measurements were performed by electrophoresis in agarose gel. CPK-BB remained unchanged in Group I during the course of the study (median range 17 to 20 IU) showing no significant difference from normal levels. Significant elevation of CPK-BB occurred three hours after establishing the ischemia in Group II (median 50 IU) as compared to Group I (p less than 0.05). The highest levels were determined to be 96 and 80 IU at the sixth and ninth hour, respectively. From hour 15 until completion of the experiment, no significant difference was detected between Groups I and II. We conclude that CPK-BB elevation in serum signifies its release from the smooth muscles of the bowel due to ischemic cellular damage.
为了确定血清中肌酸磷酸激酶脑型同工酶（CPK - BB）变化作为肠梗死酶诊断试验的用途，对患有肠缺血的犬进行了研究。该研究包括12只杂种犬。第一组（n = 6）作为对照组，对其长度为50 - 60厘米的空肠段进行肠腔阻塞。第二组（n = 6）通过结扎长度为50 - 60厘米的空肠段动脉造成肠梗死。通过左颈静脉将导管插入右心房进行采血和监测。每三小时抽取血样进行生化分析。CPK - BB测量通过琼脂糖凝胶电泳进行。在研究过程中，第一组的CPK - BB保持不变（中位数范围为17至20 IU），与正常水平无显著差异。与第一组相比，第二组在缺血建立后三小时CPK - BB显著升高（中位数为50 IU）（p小于0.05）。在第六小时和第九小时，最高水平分别确定为96 IU和80 IU。从第15小时直到实验结束，第一组和第二组之间未检测到显著差异。我们得出结论，血清中CPK - BB升高表明由于缺血性细胞损伤，其从肠平滑肌中释放出来。
