富含 Pergularia daemia 提取物的类黄酮对 CFA 诱导的关节炎大鼠的改善作用。
Amelioratory effect of flavonoids rich Pergularia daemia extract against CFA induced arthritic rats.
机构信息
Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India; TUV-SUD, South Asia Laboratory, Bangalore, 560 058, India.
出版信息
Biomed Pharmacother. 2016 May;80:244-252. doi: 10.1016/j.biopha.2016.03.019. Epub 2016 Mar 31.
PURPOSE
Pergularia daemia Forsk. (Asclepiadaceae) is a traditionally reported medicinal herb used to treat joint pain and arthritis. However, there are no scientific reports about anti-arthritic activity of P. daemia methanolic extract on rats as animal model. This study identifies bioactive compounds present in the P. daemia methanolic extract and evaluates its anti-arthritic potential in CFA induced arthritic rats.
METHODS AND RESULTS
Phytoconstituents of P. daemia extract were examined using LC-ESI/MS method. Anti-arthritic activity of P. daemia extract was determined by various biochemical experiments (RF, ESR and CRP), ultrasonography and histological analysis. LC-ESI/MS analysis resulted in the identification of major flavonoids compounds such as formononetin, qurecetin, chrysoeriol, taxifolin and naringenin. Serum biomarker analysis, after the treatment with PDME (500mg/kg b.w.) revealed that the hemoglobin (11.84±0.42g/dL) and RBC (8.38±0.67million/mm(3)) levels were significantly increased whereas WBC (8.91±0.38thousands/mm(3)), RF (17.94±0.45IU/mL), ESR (7.91±0.12mm/h) and CRP (22.56±0.26mg/L) levels were decreased when compared with the CFA induced arthritic control group. Histology results revealed that treatment with PDME has resulted in significant prevention against bony destruction by decreasing soft tissue swelling and narrowing of joint spaces (250 and 500mg/kg b.w.).
CONCLUSION
Anti-arthritic effect of P. daemia might be due to the presence of these bioactive flavonoids. These findings lend pharmacological support to the reported folkloric use of P. daemia in the treatment and management of painful, arthritic inflammatory conditions.
目的
Pergularia daemia Forsk.（萝藦科）是一种传统的药用草药，用于治疗关节疼痛和关节炎。然而，目前尚无关于 P. daemia 甲醇提取物对动物模型大鼠的抗关节炎活性的科学报道。本研究鉴定了 P. daemia 甲醇提取物中的生物活性化合物，并评估了其在 CFA 诱导的关节炎大鼠中的抗关节炎潜力。
方法和结果
使用 LC-ESI/MS 方法检查 P. daemia 提取物的植物成分。通过各种生化实验（RF、ESR 和 CRP）、超声检查和组织学分析来确定 P. daemia 提取物的抗关节炎活性。LC-ESI/MS 分析结果表明，主要的黄酮类化合物如芒柄花素、槲皮素、芹菜素、栎精和柚皮苷。用 PDME（500mg/kg b.w.）治疗后，血清生物标志物分析显示血红蛋白（11.84±0.42g/dL）和 RBC（8.38±0.67million/mm(3)）水平显著升高，而白细胞（8.91±0.38 千/mm(3)）、RF（17.94±0.45IU/mL）、ESR（7.91±0.12mm/h）和 CRP（22.56±0.26mg/L）水平降低与 CFA 诱导的关节炎对照组相比。组织学结果表明，PDME 治疗可通过减少软组织肿胀和缩小关节间隙来显著预防骨破坏（250 和 500mg/kg b.w.）。
结论
P. daemia 的抗关节炎作用可能是由于这些生物活性黄酮类化合物的存在。这些发现为 P. daemia 用于治疗和管理疼痛性关节炎炎症性疾病的民间传说用途提供了药理学支持。
Zotero 插件