Uttra Ambreen Malik, Hasan Umme Habiba
Laboratory of Cardiovascular Research and Integrative Pharmacology, Department of Pharmacology, Faculty of Pharmacy, University Of Sargodha, Sargodha, Pakistan.
BMC Complement Altern Med. 2017 Jul 18;17(1):371. doi: 10.1186/s12906-017-1879-9.
The roots and stem bark of Berberis orthobotrys (Berberidaceae) have long been used traditionally to treat joint pain. Though, it has not been pharmacologically assessed for rheumatoid arthritis. The current study explores anti-arthritic activity and phytochemical analysis of aqueous-methanolic extract (30:70) and fractions (ethyl acetate, n-butanol, and aqueous) of Berberis orthobotrys roots.
Anti-arthritic potential was evaluated in vitro using protein denaturation (bovine serum albumin and egg albumin) and membrane stabilization methods at 12.5-800 μg/ml concentration and in vivo via turpentine oil, formaldehyde and Complete Freund Adjuvant (CFA) models at 50, 100 and 150 mg/kg doses. Also, in vitro antioxidant ability was appraised by reducing power assay. Moreover, total flavonoid content, Fourier transform infrared spectroscopy and High performance liquid chromatography of n-butanol fraction were performed.
The results revealed concentration dependent inhibition of albumin denaturation and notable RBC membrane stabilization, with maximum results obtained at 800 μg/ml. Similarly, plant exhibited dose dependent anti-arthritic effect in turpentine oil and formaldehyde models, with maximum activity observed at 150 mg/kg. The results of CFA model depicted better protection against arthritic lesions and body weight alterations. Also, B.orthobotrys remarkably ameliorated altered hematological parameters, rheumatoid factor and positively modified radiographic and histopathological changes. Additionally, plant exhibited remarkable anti-oxidant activity. Moreover, phytochemical analysis revealed polyphenols and flavonoids.
Taken together, these results support traditional use of B.orthobotrys as potent anti-arthritic agent that may be proposed for rheumatoid arthritis treatment.
长叶小檗（小檗科）的根和茎皮长期以来一直被传统用于治疗关节疼痛。然而，其对类风湿性关节炎的药理作用尚未得到评估。本研究探讨了长叶小檗根的水甲醇提取物（30:70）及其馏分（乙酸乙酯、正丁醇和水相）的抗关节炎活性及植物化学分析。
通过蛋白质变性（牛血清白蛋白和蛋清白蛋白）和膜稳定化方法，在浓度为12.5 - 800μg/ml时体外评估抗关节炎潜力，并通过松节油、甲醛和完全弗氏佐剂（CFA）模型，在剂量为50、100和150mg/kg时体内评估抗关节炎潜力。此外，通过还原能力测定评估体外抗氧化能力。而且，对正丁醇馏分进行了总黄酮含量、傅里叶变换红外光谱和高效液相色谱分析。
结果显示对白蛋白变性有浓度依赖性抑制作用，且对红细胞膜有显著稳定作用，在800μg/ml时效果最佳。同样，该植物在松节油和甲醛模型中表现出剂量依赖性抗关节炎作用，在150mg/kg时活性最高。CFA模型结果显示对关节炎病变和体重变化有更好的保护作用。此外，长叶小檗显著改善了血液学参数、类风湿因子的改变，并积极改善了影像学和组织病理学变化。另外，该植物表现出显著的抗氧化活性。而且，植物化学分析显示含有多酚和黄酮类化合物。
综上所述，这些结果支持长叶小檗作为一种有效的抗关节炎药物的传统用途，可用于类风湿性关节炎的治疗。
Zotero 插件