University College London Medical School, Gower Street, London, WC1E 6BT, United Kingdom.
University of Cambridge, Addenbrooke's Hospital, Cambridge, CB2 0QQ, United Kingdom.
Curr Stem Cell Res Ther. 2017;12(6):484-492. doi: 10.2174/1574888X11666160429122133.
Since the discovery and isolation of a mesenchymal stem cell population from within the stromal vascular fraction (SVF) of adipose tissue, there has been a concerted effort to discover the characteristics of these cells. Particular attention has been paid to their morphology, selfrenewal capacity, multi-lineage differentiation capabilities and, as is of greatest interest in this instance, their cell surface profile.
The purpose of this study is to analyze and summarize the available literature that pertains to the cell surface characterization of adipose-derived stem cells (ASCs). The identification of a common set of positive and negative cell surface markers would allow for a much more consistent and reliable method of identifying this stem cell population both in vitro and in vivo.
The keywords "adipose-derived stem cells; stromal cells; surface markers" were searched in the following electronic databases: Medline, PubMed, ZETOC, Web of Knowledge, AMED, EMBASE, Ovid in process & other non-indexed citations and PsychINFO.
The most commonly reported positive markers were found to be CD90, CD44, CD29, CD105, CD13, CD34, CD73, CD166, CD10, CD49e and CD59, while the most commonly found negative markers were CD31, CD45, CD14, CD11b, CD34, CD19, CD56 and CD146. In addition, a number of other markers appeared in the literature including HLA-ABC, HLA-DR, SH2, SH3, STRO-1, VEGF2, vWF, ABCG2, SSEA-1 (CD15), PDGFR, alpha- SMA, c-Kit (CD117), OCT4+ and CCR5X (CD195).
A minimum panel of positive and negative markers for identifying ASCs can be recommended. The following markers should be positive: CD90, CD44, CD29, CD105, CD13, CD34, CD73, CD166, CD10, CD49e and CD59. The following markers should be negative: CD31, CD45, CD14, CD11b, CD19, CD56 and CD146. In addition to this, the positive expression of HLA- ABC and STRO- 1 should be seen along with the negative expression on HLA-DR. This review, however, has also found that there are a number of disagreements over the expression and existence of various markers, namely CD31, CD34, c- Kit (CD117) and STRO-1.
自从从脂肪组织的基质血管部分（SVF）中发现并分离出间充质干细胞群以来，人们一直在努力发现这些细胞的特性。特别关注的是它们的形态、自我更新能力、多谱系分化能力，以及在这种情况下最感兴趣的是它们的细胞表面特征。
本研究旨在分析和总结与脂肪来源干细胞（ASCs）的细胞表面特征相关的现有文献。鉴定一组共同的阳性和阴性细胞表面标志物，将允许在体外和体内更一致和可靠地识别这种干细胞群体。
在以下电子数据库中搜索关键词“脂肪来源干细胞；基质细胞；表面标志物”：Medline、PubMed、ZETOC、Web of Knowledge、AMED、EMBASE、ovid 正在处理和其他非索引引文和 PsychINFO。
发现最常报告的阳性标记物为 CD90、CD44、CD29、CD105、CD13、CD34、CD73、CD166、CD10、CD49e 和 CD59，而最常发现的阴性标记物为 CD31、CD45、CD14、CD11b、CD34、CD19、CD56 和 CD146。此外，文献中还出现了一些其他标记物，包括 HLA-ABC、HLA-DR、SH2、SH3、STRO-1、VEGF2、vWF、ABCG2、SSEA-1（CD15）、PDGFR、alpha-SMA、c-Kit（CD117）、OCT4+和 CCR5X（CD195）。
可以推荐一组用于识别 ASC 的阳性和阴性标志物的最小面板。以下标记物应为阳性：CD90、CD44、CD29、CD105、CD13、CD34、CD73、CD166、CD10、CD49e 和 CD59。以下标记物应为阴性：CD31、CD45、CD14、CD11b、CD19、CD56 和 CD146。除此之外，还应看到 HLA-ABC 的阳性表达和 STRO-1 的阳性表达，以及 HLA-DR 的阴性表达。然而，本综述还发现，对于各种标记物的表达和存在存在许多分歧，即 CD31、CD34、c-Kit（CD117）和 STRO-1。
