Tay S W, Merrill E W, Salzman E W, Lindon J
Department of Chemical Engineering, M.I.T., Cambridge, MA.
Biomaterials. 1989 Jan;10(1):11-5. doi: 10.1016/0142-9612(89)90003-3.
Commercially obtained (Diosynth) heparin was covalently bonded to poly (vinyl alcohol) (PVA) hydrogels and to polyethylene oxide (PEO) hydrogels activated by tresyl chloride. We found that as tresyl chloride activation of PVA increased, the specific activity of the bound heparin toward thrombin and antithrombin decreased by nearly a factor of 10 and that commercial heparin bound to PEO had nearly ten-fold greater activity than when bound to PVA at comparable concentrations. These findings suggest that the long 'leash' provided by PEO hydrogels may give the heparin more access to the thrombin-antithrombin pair than the tight bond to PVA, and that crowding of heparin units on a surface limits access of the thrombin-antithrombin pair.
市售（迪奥斯ynth）肝素通过共价键结合到经三氟甲磺酸氯活化的聚乙烯醇（PVA）水凝胶和聚环氧乙烷（PEO）水凝胶上。我们发现，随着PVA经三氟甲磺酸氯活化程度的增加，结合的肝素对凝血酶和抗凝血酶的比活性降低了近10倍，并且在可比浓度下，结合到PEO的市售肝素的活性比结合到PVA时高近10倍。这些发现表明，PEO水凝胶提供的长“链”可能使肝素比与PVA的紧密结合更易接近凝血酶-抗凝血酶对，并且肝素单元在表面的拥挤限制了凝血酶-抗凝血酶对的接近。
