Řehulka J, Kubátová A, Hubka V
Department of Zoology, Silesian Museum, Opava, Czech Republic.
Department of Botany, Faculty of Science, Charles University in Prague, Prague 2, Czech Republic.
J Fish Dis. 2016 Dec;39(12):1413-1419. doi: 10.1111/jfd.12477. Epub 2016 May 2.
The first case of visceral mycotic infection due to Cephalotheca sulfurea (Cephalothecaceae, Ascomycota) is documented in farmed rainbow trout from a raceway culture system. The disease clinically manifested as a hyperaemic area in the liver of the fish, and histological examination using silver and PAS staining showed the presence of numerous foci of hyphae and spores. The causative agent was first isolated in pure culture from the liver and identified using morphological characteristics. Sequence data from ITS and LSU rDNA also clearly confirmed C. sulfurea as the causal agent. The pathogenicity of related species belonging to the family Cephalothecaceae has been well-documented in humans and dogs (superficial as well as systemic infections). However, C. sulfurea has never been reported as a pathogen of humans or animals, including marine and freshwater fishes. The morphological identification of C. sulfurea is difficult due to its similarity to several different fungal genera, and molecular methods are strongly recommended for reliable identification.
在一个流水养殖系统的养殖虹鳟鱼中，记录了首例由硫磺头孢霉（头孢霉科，子囊菌门）引起的内脏霉菌感染病例。该病在鱼的肝脏中临床表现为充血区域，使用银染和PAS染色的组织学检查显示存在大量菌丝和孢子病灶。病原体首先从肝脏中分离出来进行纯培养，并根据形态特征进行鉴定。来自ITS和LSU rDNA的序列数据也明确证实硫磺头孢霉为病原体。头孢霉科相关物种在人类和犬类（包括浅表和全身感染）中的致病性已有充分记录。然而，硫磺头孢霉从未被报道为人类或动物（包括海洋和淡水鱼类）的病原体。硫磺头孢霉的形态鉴定因其与几个不同真菌属相似而困难，强烈建议使用分子方法进行可靠鉴定。
