Wang Mian, Chen Mingna, Yang Zhen, Chen Na, Chi Xiaoyuan, Pan Lijuan, Wang Tong, Yu Shanlin, Guo Xingqi
State Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University, Taian 271018, China.
Shandong Peanut Research Institute, Qingdao 266100, China.
Mycobiology. 2017 Dec;45(4):392-400. doi: 10.5941/MYCO.2017.45.4.392. Epub 2017 Dec 31.
Peanut yield and quality are seriously affected by pod rot pathogens worldwide, especially in China in recent years. The goals of this study are to analyze the structure of fungal communities of peanut pod rot in soil in three peanut cultivars and the correlation of pod rot with environmental variables using 454 pyrosequencing. A total of 46,723 internal transcribed spacer high-quality sequences were obtained and grouped into 1,706 operational taxonomic units at the 97% similarity cut-off level. The coverage, rank abundance, and the Chao 1 and Shannon diversity indices of the operational taxonomic units were analyzed. Members of the phylum Ascomycota were dominant, such as , , , and , followed by Basidiomycota. The results of the heatmap and redundancy analysis revealed significant variation in the composition of the fungal community among the three cultivar samples. The environmental conditions in different peanut cultivars may also influence on the structure of the fungal community. The results of this study suggest that the causal agent of peanut pod rot may be more complex, and cultivars and environmental conditions are both important contributors to the community structure of peanut pod rot fungi.
在全球范围内，尤其是近年来在中国，花生荚腐病菌严重影响花生的产量和品质。本研究的目的是利用454焦磷酸测序技术分析三个花生品种土壤中花生荚腐病真菌群落的结构，以及荚腐病与环境变量之间的相关性。共获得46723条高质量的内转录间隔区序列，并在97%的相似性截止水平上分为1706个操作分类单元。分析了操作分类单元的覆盖率、秩丰度以及Chao 1和Shannon多样性指数。子囊菌门的成员占主导地位，如 、 、 和 ，其次是担子菌门。热图和冗余分析结果显示，三个品种样品的真菌群落组成存在显著差异。不同花生品种的环境条件也可能影响真菌群落的结构。本研究结果表明，花生荚腐病的病原菌可能更为复杂，品种和环境条件都是花生荚腐病真菌群落结构的重要影响因素。
