Nakamura A, Sugao T, Yamaue K, Kobatake M, Shiomi H
Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Japan.
Brain Res. 1989 Feb 20;480(1-2):82-6. doi: 10.1016/0006-8993(89)91569-2.
We studied whether the antinociceptive effect produced by intracerebroventricular injection of delta-sleep-inducing peptide (DSIP) to mice involved the monoaminergic pathways that descended from brainstem to spinal cord (the descending inhibitory systems). In the tail-pinch test, the antinociceptive effect of DSIP was significantly reduced by the pretreatment with reserpine (3 mg/kg i.p.) which depleted endogenous monoamines. Moreover, the intrathecal injections of monoamine antagonists were performed to evaluate the roles of the spinal noradrenergic and/or serotonergic systems in the production of the DSIP antinociception. In both tail-pinch and hot plate tests, the antinociceptive effect of DSIP was significantly antagonized by the previous intrathecal administration of phentolamine (an alpha-adrenergic blocker) or yohimbine (an alpha 2-adrenergic blocker), but was unaffected by the pretreatment with methysergide (a serotonin antagonist). These results demonstrate that the activation of the descending inhibitory systems, mainly spinal noradrenergic systems, is involved in the elicitation of DSIP antinociception.
我们研究了向小鼠脑室内注射δ-睡眠诱导肽（DSIP）所产生的抗伤害感受作用是否涉及从脑干下行至脊髓的单胺能通路（下行抑制系统）。在夹尾试验中，用利血平（3毫克/千克腹腔注射）进行预处理可耗尽内源性单胺，从而显著降低DSIP的抗伤害感受作用。此外，进行鞘内注射单胺拮抗剂以评估脊髓去甲肾上腺素能和/或5-羟色胺能系统在DSIP抗伤害感受产生中的作用。在夹尾试验和热板试验中，鞘内预先注射酚妥拉明（一种α-肾上腺素能阻滞剂）或育亨宾（一种α2-肾上腺素能阻滞剂）可显著拮抗DSIP的抗伤害感受作用，但用美西麦角（一种5-羟色胺拮抗剂）进行预处理则无影响。这些结果表明，下行抑制系统，主要是脊髓去甲肾上腺素能系统的激活，参与了DSIP抗伤害感受的引发。
