Khan Hammad A, Siddique Kadambot H M, Colmer Timothy D
School of Plant Biology, Faculty of Science, The University of Western Australia, 35 Stirling Hwy, Crawley WA 6009, Australia.
The UWA Institute of Agriculture, The University of Western Australia, 35 Stirling Hwy, Crawley WA 6009, Australia.
J Exp Bot. 2017 Apr 1;68(8):2001-2011. doi: 10.1093/jxb/erw177.
Reproductive processes of chickpea (Cicer arietinum L.) are particularly sensitive to salinity. We tested whether limited photoassimilate availability contributes to reproductive failure in salt-stressed chickpea. Rupali, a salt-sensitive genotype, was grown in aerated nutrient solution, either with non-saline (control) or 30mM NaCl treatment. At flowering, stems were either infused with sucrose solution (0.44M), water only or maintained without any infusion, for 75 d. The sucrose and water infusion treatments of non-saline plants had no effect on growth or yield, but photosynthesis declined in response to sucrose infusion. Salt stress reduced photosynthesis, decreased tissue sugars by 22-47%, and vegetative and reproductive growth were severely impaired. Sucrose infusion of salt-treated plants increased total sugars in stems, leaves and developing pods, to levels similar to those of non-saline plants. In salt-stressed plants, sucrose infusion increased dry mass (2.6-fold), pod numbers (3.8-fold), seed numbers (6.5-fold) and seed yield (10.4-fold), yet vegetative growth and reproductive failure were not rescued completely by sucrose infusion. Sucrose infusion partly rescued reproductive failure in chickpea by increasing vegetative growth enabling more flower production and by providing sucrose for pod and seed growth. We conclude that insufficient assimilate availability limits yield in salt-stressed chickpea.
鹰嘴豆（Cicer arietinum L.）的生殖过程对盐分特别敏感。我们测试了有限的光合产物供应是否会导致盐胁迫下鹰嘴豆的生殖失败。盐敏感基因型鲁帕利在通气营养液中生长，分别进行无盐（对照）或30mM NaCl处理。在开花期，对茎干进行处理，分别注入蔗糖溶液（0.44M）、仅注入水或不进行任何注入，持续75天。无盐处理植株的蔗糖和水注入处理对生长或产量没有影响，但蔗糖注入会使光合作用下降。盐胁迫降低了光合作用，使组织糖分减少22%-47%，营养生长和生殖生长均受到严重损害。对盐处理植株注入蔗糖可使茎、叶和发育中的豆荚中的总糖分增加，达到与无盐处理植株相似的水平。在盐胁迫植株中，注入蔗糖使干重增加了2.6倍，豆荚数量增加了3.8倍，种子数量增加了6.5倍，种子产量增加了10.4倍，但注入蔗糖并未完全挽救营养生长和生殖失败的情况。注入蔗糖通过促进营养生长从而产生更多花朵，并为豆荚和种子生长提供蔗糖，部分挽救了鹰嘴豆的生殖失败。我们得出结论，光合产物供应不足限制了盐胁迫下鹰嘴豆的产量。
