Shuai Jianjun, Yu Xurun, Zhang Jing, Xiong Ai-Sheng, Xiong Fei
Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China.
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Weigang, Nanjing, China.
Braz J Microbiol. 2016 Jul-Sep;47(3):536-41. doi: 10.1016/j.bjm.2014.12.001. Epub 2016 Apr 22.
Polychlorinated biphenyls (PCBs), the chlorinated derivatives of biphenyl, are one of the most prevalent, highly toxic and persistent groups of contaminants in the environment. The objective of this study was to investigate the biodegradation of PCBs in northeastern (Heilongjiang Province), northern (Shanxi Province) and eastern China (Shanghai municipality). From these areas, nine soil samples were screened for PCB-degrading bacteria using a functional complementarity method. The genomic 16S rDNA locus was amplified and the products were sequenced to identify the bacterial genera. Seven Pseudomonas strains were selected to compare the capacity of bacteria from different regions to degrade biphenyl by HPLC. Compared to the biphenyl content in controls of 100%, the biphenyl content went down to 3.7% for strain P9-324, 36.3% for P2-11, and 20.0% for the other five strains. These results indicate that a longer processing time led to more degradation of biphenyl. PCB-degrading bacterial strains are distributed differently in different regions of China.
多氯联苯（PCBs）是联苯的氯化衍生物，是环境中最普遍、毒性最强且持久的污染物类别之一。本研究的目的是调查中国东北（黑龙江省）、北部（山西省）和东部（上海市）地区多氯联苯的生物降解情况。从这些地区采集了9份土壤样本，采用功能互补法筛选多氯联苯降解菌。扩增基因组16S rDNA位点并对产物进行测序以鉴定细菌属。选择7株假单胞菌菌株，通过高效液相色谱法比较不同地区细菌降解联苯的能力。与对照中联苯含量的100%相比，菌株P9 - 324的联苯含量降至3.7%，P2 - 11为36.3%，其他5株为20.0%。这些结果表明，处理时间越长，联苯降解得越多。多氯联苯降解菌株在中国不同地区的分布有所不同。
