College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, China.
Curr Microbiol. 2010 Nov;61(5):477-83. doi: 10.1007/s00284-010-9641-2. Epub 2010 Apr 20.
As one of China's great metropolises, Shanghai is vulnerable to various forms of industrial and agricultural contamination associated with its development. Polychlorinated biphenyls (PCBs) are man-made chemicals that never existed in nature until the 1900s when they started to be released into the environment. PCBs are hazardous environmental contaminants that bind strongly to soil. In this study, four soil samples were screened for the presence of PCB-degrading bacteria. The 16 S rDNAs were amplified from those genomes and the products (~1.5 kb) were purified and sequenced for the isolation and identification of bacterial species. Four Pseudomonas strains (strain 1-212 from sample 1; strain 2-241 from sample 2; strain 3-318 from sample 3; and strain 4-150 from sample 4) were selected for analysis by HPLC. Setting the content of the biphenyl in CK as 100%, the biphenyl contents was 2.32% in 1-212, 73.11% in 2-241, 69.83% in 3-318, and 86.16% in 4-150. The results of this study suggest directions for future research, including genetic screening, cloning and restructuring, and provide guidance for the cultivation of PCBs-degrading bacteria.
作为中国的一个大都市，上海容易受到与发展相关的各种形式的工农业污染。多氯联苯（PCBs）是人工合成的化学物质，直到 20 世纪才开始释放到环境中，在此之前它们在自然界中从未存在过。PCBs 是危险的环境污染物，它们与土壤强烈结合。在这项研究中，筛选了四个土壤样本中存在的可降解 PCB 的细菌。从这些基因组中扩增了 16S rDNA，并对产物（约 1.5kb）进行了纯化和测序，以分离和鉴定细菌种类。选择了四个假单胞菌菌株（来自样本 1 的 1-212 株；来自样本 2 的 2-241 株；来自样本 3 的 3-318 株；以及来自样本 4 的 4-150 株）进行 HPLC 分析。将 CK 中二苯并的含量设定为 100%，则 1-212 中二苯并的含量为 2.32%，2-241 中二苯并的含量为 73.11%，3-318 中二苯并的含量为 69.83%，4-150 中二苯并的含量为 86.16%。本研究的结果为未来的研究提供了方向，包括遗传筛选、克隆和重组，并为 PCB 降解菌的培养提供了指导。
