从人扁桃体和淋巴结中纯化树突状细胞亚群
Dendritic Cell Subset Purification from Human Tonsils and Lymph Nodes.
作者信息
Durand Mélanie, Segura Elodie
机构信息
Institut Curie, Centre de recherche, 26 rue d'Ulm, 75248, Paris Cedex 05, France.
INSERM U932, 26 rue d'Ulm, 75248, Paris Cedex 05, France.
出版信息
Methods Mol Biol. 2016;1423:89-99. doi: 10.1007/978-1-4939-3606-9_6.
PMID:27142010
Abstract
Dendritic cells (DCs) are a rare population of antigen-presenting cells that initiate immune responses in secondary lymphoid organs. In order to better understand the properties of DC in humans, it is essential to analyze DC subsets directly purified from tissues. Here, we describe a protocol allowing the purification of DC subsets from human tonsils and human lymph nodes.
摘要
树突状细胞（DCs）是一类罕见的抗原呈递细胞，可在次级淋巴器官中启动免疫反应。为了更好地了解人类DC的特性，直接分析从组织中纯化的DC亚群至关重要。在此，我们描述了一种从人扁桃体和人淋巴结中纯化DC亚群的方法。
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