Department of ORL, Head & Neck Surgery, Skåne University Hospital, Lund, Sweden; Department of Clinical Sciences, Lund University, Lund, Sweden.
Department of Immunotechnology, Lund University, Lund, Sweden.
Hum Immunol. 2021 Dec;82(12):976-981. doi: 10.1016/j.humimm.2021.08.007. Epub 2021 Sep 9.
Dendritic cells (DCs) with capacity of antigen cross-presentation are of key interest for immunotherapy against cancer as they can induce antigen-specific cytotoxic T lymphocyte (CTL) responses. This study describes frequencies of DC subtypes in human tonsils and lymph nodes, and phenotypic aspects that may be targeted by adjuvant measures. From human tonsils and neck lymph nodes, DCs were identified through flow cytometry, and subsets of plasmacytoid DCs (pDCs) and myeloid DCs (mDCs) were investigated. Maturity status was assessed and surface receptors with CTL-promoting potentials were studied. CD123 pDCs as well as CD1c, CD141, and CD1cCD141 mDCs were detected in tonsils and lymph nodes. Both sites featured a similar presence of DC subsets, with CD123 pDC being dominant and CD141 mDCs least frequent. Based on CD80/CD86 expression, all DC subtypes featured a low degree of maturation. Expression of pattern recognition receptors (PRRs) CD206, CD207, DC-SIGN, TLR2, and TLR4, as well as the chemokine receptor XCR1, indicated DC subset-specific receptor profiles. We conclude that tonsils and lymph nodes share common features in terms of DC subset frequency and maturation as well as PRR and XCR1 expression pattern. Our work suggests that both sites may be considered for vaccine deposition in DC-mediated immunotherapy.
树突状细胞（DCs）具有抗原交叉呈递能力，是癌症免疫治疗的关键，因为它们可以诱导抗原特异性细胞毒性 T 淋巴细胞（CTL）反应。本研究描述了人扁桃体和淋巴结中 DC 亚型的频率，以及可能被佐剂措施靶向的表型特征。通过流式细胞术从人扁桃体和颈部淋巴结中鉴定出 DC，并研究了浆细胞样 DC（pDC）和髓样 DC（mDC）的亚群。评估了成熟状态，并研究了具有 CTL 促进潜力的表面受体。在扁桃体和淋巴结中均检测到 CD123 pDC 以及 CD1c、CD141 和 CD1cCD141 mDC。两个部位的 DC 亚群存在相似，其中 CD123 pDC 占主导地位，CD141 mDC 最少。基于 CD80/CD86 的表达，所有 DC 亚型的成熟程度都较低。模式识别受体（PRRs）CD206、CD207、DC-SIGN、TLR2 和 TLR4 的表达以及趋化因子受体 XCR1 的表达表明了 DC 亚群特异性的受体谱。我们得出结论，扁桃体和淋巴结在 DC 亚群频率和成熟度以及 PRR 和 XCR1 表达模式方面具有共同特征。我们的工作表明，这两个部位都可以被认为是用于疫苗在 DC 介导的免疫治疗中沉积的部位。
