从淋巴结和扁桃体中纯化人类自然杀伤细胞发育中间体。

Purification of human NK cell developmental intermediates from lymph nodes and tonsils.

作者信息

Freud Aharon G, Caligiuri Michael A

机构信息

Department of Pathology, Stanford University, Stanford, CA, USA.

出版信息

Methods Mol Biol. 2010;612:1-14. doi: 10.1007/978-1-60761-362-6_1.

DOI:10.1007/978-1-60761-362-6_1

PMID:20033630

Abstract

Accumulating data indicate that human natural killer (NK) cells undergo terminal maturation in secondary lymphoid tissues (SLTs) including lymph nodes (LNs) and tonsils. In addition, recent studies have revealed that maturing NK cells progress through at least five functionally discrete stages of development within SLTs. These discoveries provide unique possibilities for researchers to investigate the natural processes governing human NK cell development, as they exist in vivo, through analysis of NK cell maturational intermediates found in situ. Herein we describe a detailed, yet simple, four-step protocol for the viable enrichment and purification of human NK cell developmental intermediates from LNs and tonsils.

摘要

越来越多的数据表明，人类自然杀伤（NK）细胞在包括淋巴结（LNs）和扁桃体在内的二级淋巴组织（SLTs）中经历终末成熟。此外，最近的研究表明，成熟的NK细胞在SLTs内至少经历五个功能上不同的发育阶段。这些发现为研究人员提供了独特的机会，通过分析原位发现的NK细胞成熟中间体，来研究体内存在的人类NK细胞发育的自然过程。在此，我们描述了一个详细但简单的四步方案，用于从LNs和扁桃体中对人类NK细胞发育中间体进行活细胞富集和纯化。