Holst O, Christoffel V, Fründ R, Moll H, Sumper M
Lehrstuhl Biochemie I der Universität, Regensburg, Federal Republic of Germany.
Eur J Biochem. 1989 May 1;181(2):345-50. doi: 10.1111/j.1432-1033.1989.tb14730.x.
The sulphated glycoprotein SSG 185 is the monomeric precursor of a highly aggregated structural element in the extracellular matrix of the multicellular green alga Volvox carteri. A phosphodiester of arabinose was isolated from a saccharide fragment of SSG 185. The structure of this phosphodiester was investigated by methylation analysis, 13C-NMR, photometric methods and enzymatic assays and identified as D-Araiota-5-phospho-5-D-Araiota. The function of this phosphodiester bridge as a crosslink of different carbohydrate chains in SSG 185 is discussed.
硫酸化糖蛋白SSG 185是多细胞绿藻团藻细胞外基质中高度聚集的结构元件的单体前体。从SSG 185的一个糖片段中分离出阿拉伯糖的磷酸二酯。通过甲基化分析、¹³C-NMR、光度法和酶促测定研究了该磷酸二酯的结构，并鉴定为D-阿拉伯糖-5-磷酸-5-D-阿拉伯糖。讨论了这种磷酸二酯桥作为SSG 185中不同碳水化合物链交联的功能。
