团藻的细胞外基质：细胞区室的分子结构
The extracellular matrix of Volvox carteri: molecular structure of the cellular compartment.
作者信息
Ertl H, Mengele R, Wenzl S, Engel J, Sumper M
机构信息
Lehrstuhl Biochemie I, Universität Regensburg, Federal Republic of Germany.
出版信息
J Cell Biol. 1989 Dec;109(6 Pt 2):3493-501. doi: 10.1083/jcb.109.6.3493.
Abstract
The extracellular matrix (ECM) of Volvox contains insoluble fibrous layers that surround individual cells at a distance to form contiguous cellular compartments. Using immunological techniques, we identified a sulfated surface glycoprotein (SSG 185) as the monomeric precursor of this substructure within the ECM. The primary structure of the SSG 185 poly-peptide chain has been derived from cDNA and genomic DNA. A central domain of the protein, 80 amino acid residues long, consists almost exclusively of hydroxyproline residues. The chemical structure of the highly sulfated polysaccharide covalently attached to SSG 185 has been determined by permethylation analysis. As revealed by EM, SSG 185 is a rod-shaped molecule with a 21-nm-long polysaccharide strand protruding from its central region. The chemical nature of the cross-links between SSG 185 monomers is discussed.
摘要
团藻的细胞外基质（ECM）包含不溶性纤维层，这些纤维层在一定距离围绕单个细胞，形成连续的细胞区室。利用免疫学技术，我们鉴定出一种硫酸化表面糖蛋白（SSG 185）是ECM中这种亚结构的单体前体。SSG 185多肽链的一级结构已从cDNA和基因组DNA推导得出。该蛋白的一个中央结构域长80个氨基酸残基，几乎完全由羟脯氨酸残基组成。通过全甲基化分析确定了与SSG 185共价连接的高度硫酸化多糖的化学结构。如电子显微镜所示，SSG 185是一种杆状分子，有一条21纳米长的多糖链从其中心区域伸出。文中讨论了SSG 185单体之间交联的化学性质。
相似文献
1
The extracellular matrix of Volvox carteri: molecular structure of the cellular compartment.团藻的细胞外基质：细胞区室的分子结构
J Cell Biol. 1989 Dec;109(6 Pt 2):3493-501. doi: 10.1083/jcb.109.6.3493.
2
A novel extensin that may organize extracellular matrix biogenesis in Volvox carteri.一种可能在团藻中组织细胞外基质生物合成的新型伸展蛋白。
EMBO J. 1992 Jun;11(6):2055-62. doi: 10.1002/j.1460-2075.1992.tb05263.x.
3
Biochemistry of the extracellular matrix of Volvox.团藻细胞外基质的生物化学
Int Rev Cytol. 1998;180:51-85. doi: 10.1016/s0074-7696(08)61770-2.
4
A phosphodiester bridge between two arabinose residues as a structural element of an extracellular glycoprotein of Volvox carteri.作为团藻胞外糖蛋白结构元件的两个阿拉伯糖残基之间的磷酸二酯桥。
Eur J Biochem. 1989 May 1;181(2):345-50. doi: 10.1111/j.1432-1033.1989.tb14730.x.
5
Evidence for autocatalytic cross-linking of hydroxyproline-rich glycoproteins during extracellular matrix assembly in Volvox.关于团藻细胞外基质组装过程中富含羟脯氨酸糖蛋白的自催化交联的证据。
Plant Cell. 2002 May;14(5):1147-60. doi: 10.1105/tpc.000711.
6
7
Response to the sexual pheromone and wounding in the green alga volvox: induction of an extracellular glycoprotein consisting almost exclusively of hydroxyproline.绿藻团藻对性信息素和创伤的反应：诱导产生一种几乎仅由羟脯氨酸组成的细胞外糖蛋白。
J Biol Chem. 1999 Dec 3;274(49):35023-8. doi: 10.1074/jbc.274.49.35023.
8
Differential targeting of closely related ECM glycoproteins: the pherophorin family from Volvox.密切相关的细胞外基质糖蛋白的差异靶向：来自团藻属的pherophorin家族。
EMBO J. 1997 Jan 2;16(1):25-34. doi: 10.1093/emboj/16.1.25.
9
Transcriptional activation by the sexual pheromone and wounding: a new gene family from Volvox encoding modular proteins with (hydroxy)proline-rich and metalloproteinase homology domains.性信息素和创伤诱导的转录激活：来自团藻的一个新基因家族，其编码具有富含（羟基）脯氨酸和金属蛋白酶同源结构域的模块化蛋白。
Plant J. 2001 Jun;26(6):583-93. doi: 10.1046/j.1365-313x.2001.01059.x.
10
Cell Type-Specific Pherophorins of Reveal Interplay of Both Cell Types in ECM Biosynthesis.细胞类型特异性pherophorins 的揭示了细胞类型在细胞外基质生物合成中的相互作用。
Cells. 2022 Dec 29;12(1):134. doi: 10.3390/cells12010134.
引用本文的文献
1
Spatiotemporal distribution of the glycoprotein pherophorin II reveals stochastic geometry of the growing ECM of .糖蛋白pherophorin II的时空分布揭示了……生长的细胞外基质的随机几何结构。 （原文结尾不完整，翻译可能会稍有不通顺）
Proc Natl Acad Sci U S A. 2025 Aug 19;122(33):e2425759122. doi: 10.1073/pnas.2425759122. Epub 2025 Aug 12.
2
The microalga as a cell supportive building block for tissue engineering.微藻作为组织工程中细胞支持性构建单元。
Mater Today Bio. 2024 Feb 29;25:101013. doi: 10.1016/j.mtbio.2024.101013. eCollection 2024 Apr.
3
Cell Type-Specific Pherophorins of Reveal Interplay of Both Cell Types in ECM Biosynthesis.细胞类型特异性pherophorins 的揭示了细胞类型在细胞外基质生物合成中的相互作用。
Cells. 2022 Dec 29;12(1):134. doi: 10.3390/cells12010134.
4
Whole transcriptome RNA-Seq analysis reveals extensive cell type-specific compartmentalization in Volvox carteri.全转录组 RNA-Seq 分析揭示了衣藻中广泛的细胞类型特异性区室化。
BMC Biol. 2017 Nov 28;15(1):111. doi: 10.1186/s12915-017-0450-y.
5
Genomics of Volvocine Algae.团藻目藻类的基因组学
Adv Bot Res. 2012;64:185-243. doi: 10.1016/B978-0-12-391499-6.00006-2.
6
Multicellularity in green algae: upsizing in a walled complex.绿藻中的多细胞性：在有壁复合体中的增大
Front Plant Sci. 2014 Nov 18;5:649. doi: 10.3389/fpls.2014.00649. eCollection 2014.
7
Chlamydomonas reinhardtii has multiple prolyl 4-hydroxylases, one of which is essential for proper cell wall assembly.莱茵衣藻有多种脯氨酰4-羟化酶，其中一种对细胞壁的正常组装至关重要。
Plant Cell. 2007 Jan;19(1):256-69. doi: 10.1105/tpc.106.042739. Epub 2007 Jan 12.
8
Quantitative analysis of cell-type specific gene expression in the green alga Volvox carteri.绿藻团藻中细胞类型特异性基因表达的定量分析。
BMC Genomics. 2006 Dec 21;7:321. doi: 10.1186/1471-2164-7-321.
9
Sex as a response to oxidative stress: stress genes co-opted for sex.性作为对氧化应激的一种反应：应激基因被用于性方面。
Proc Biol Sci. 2005 Sep 22;272(1575):1935-40. doi: 10.1098/rspb.2005.3151.
10
Mating-induced shedding of cell walls, removal of walls from vegetative cells, and osmotic stress induce presumed cell wall genes in Chlamydomonas.交配诱导的细胞壁脱落、营养细胞的细胞壁去除以及渗透胁迫会诱导衣藻中假定的细胞壁基因。
Plant Physiol. 2005 Oct;139(2):999-1014. doi: 10.1104/pp.105.065037. Epub 2005 Sep 23.
本文引用的文献
1
Asparaginylglucose: Novel type of carbohydrate linkage.天冬氨酰葡糖：新型碳水化合物键。
Proc Natl Acad Sci U S A. 1983 Sep;80(18):5470-4. doi: 10.1073/pnas.80.18.5470.
2
Development-dependent modification of the extracellular matrix by a sulphated glycoprotein in Volvox carteri.水螅球藻中硫酸化糖蛋白对细胞外基质的发育依赖性修饰。
EMBO J. 1984 Apr;3(4):739-44. doi: 10.1002/j.1460-2075.1984.tb01877.x.
3
4
Characterization of a purified cell surface glycoprotein as a contact site in Polysphondylium pallidum.一种纯化的细胞表面糖蛋白作为苍白聚孢黏菌接触位点的表征
Exp Cell Res. 1981 Jul;134(1):181-91. doi: 10.1016/0014-4827(81)90475-4.
5
Stage-specific antigens reacting with monoclonal antibodies against contact site A, a cell-surface glycoprotein of Dictyostelium discoideum.与针对盘基网柄菌细胞表面糖蛋白接触位点A的单克隆抗体发生反应的阶段特异性抗原。
Cell Differ. 1982 Jan;11(1):1-13. doi: 10.1016/0045-6039(82)90011-2.
6
An improved procedure for the methylation analysis of oligosaccharides and polysaccharides.一种改进的寡糖和多糖甲基化分析方法。
Carbohydr Res. 1984 Apr 2;127(1):59-73. doi: 10.1016/0008-6215(84)85106-x.
7
Lambda replacement vectors carrying polylinker sequences.携带多克隆位点序列的λ置换载体。
J Mol Biol. 1983 Nov 15;170(4):827-42. doi: 10.1016/s0022-2836(83)80190-9.
8
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.pUC质粒，一种源自M13mp7的用于插入诱变和使用合成通用引物进行测序的系统。
Gene. 1982 Oct;19(3):259-68. doi: 10.1016/0378-1119(82)90015-4.
9
Use of glutaraldehyde as a coupling agent for proteins and peptides.戊二醛作为蛋白质和肽的偶联剂的应用。
Methods Enzymol. 1980;70(A):159-65. doi: 10.1016/s0076-6879(80)70047-2.
10
A simple and very efficient method for generating cDNA libraries.一种简单且非常有效的生成cDNA文库的方法。
Gene. 1983 Nov;25(2-3):263-9. doi: 10.1016/0378-1119(83)90230-5.
Zotero 插件