Smad3和磷酸化Smad3是侵袭性无功能垂体腺瘤的潜在标志物。
Smad3 and phospho-Smad3 are potential markers of invasive nonfunctioning pituitary adenomas.
作者信息
Liu Chunhui, Li Zhenye, Wu Dan, Li Chuzhong, Zhang Yazhuo
机构信息
Beijing Neurosurgical Institute, Capital Medical University, Beijing, People's Republic of China; Beijing Institute for Brain Disorders, Brain Tumor Center, Beijing, People's Republic of China.
Beijing Neurosurgical Institute, Capital Medical University, Beijing, People's Republic of China; Beijing Institute for Brain Disorders, Brain Tumor Center, Beijing, People's Republic of China; Department of Neurosurgery, Beijing Tiantan hospital, Capital Medical University, Beijing, People's Republic of China.
出版信息
Onco Targets Ther. 2016 Apr 15;9:2265-71. doi: 10.2147/OTT.S99699. eCollection 2016.
BACKGROUND
Transforming growth factor-β (TGF-β) signaling plays important roles in tumor development. Nevertheless, the roles of TGF-β/Smad signaling in nonfunctioning pituitary adenomas (NFPAs) have not been fully studied.
METHODS
Tumor samples were obtained from patients who had NFPAs and underwent endoscopic transsphenoidal surgery or craniotomy at Beijing Tiantan Hospital from March 2008 to December 2012. Immunohistochemistry was performed to determine the expression of Smad transducer proteins in NFPAs. Ki-67 was evaluated as an indicator of the proliferative activity of NFPAs.
RESULTS
A total of 161 patients with NFPAs were identified; 59 (36.6%) had invasive NFPAs and 102 (63.4%) had noninvasive NFPAs. Protein levels of Smad3 and phospho-Smad3 (p-Smad3) were significantly lower in patients with invasive NFPAs than in patients with noninvasive NFPAs (P<0.05 and P<0.01, respectively). The Ki-67 index was markedly greater in invasive NFPAs than in noninvasive NFPAs (P<0.05) and was significant correlated with p-Smad3 levels (P<0.05, r=-0.702).
CONCLUSION
A low level of Smad3 and p-Smad3 proteins was associated with the invasion of NFPAs.
背景
转化生长因子-β（TGF-β）信号传导在肿瘤发展中起重要作用。然而，TGF-β/Smad信号传导在无功能垂体腺瘤（NFPA）中的作用尚未得到充分研究。
方法
从2008年3月至2012年12月在北京天坛医院接受内镜经蝶窦手术或开颅手术的NFPA患者中获取肿瘤样本。进行免疫组织化学以确定NFPA中Smad转导蛋白的表达。评估Ki-67作为NFPA增殖活性的指标。
结果
共鉴定出161例NFPA患者；59例（36.6%）为侵袭性NFPA，102例（63.4%）为非侵袭性NFPA。侵袭性NFPA患者的Smad3和磷酸化Smad3（p-Smad3）蛋白水平显著低于非侵袭性NFPA患者（分别为P<0.05和P<0.01）。侵袭性NFPA的Ki-67指数明显高于非侵袭性NFPA（P<0.05），且与p-Smad3水平显著相关（P<0.05，r=-0.702）。
结论
低水平的Smad3和p-Smad3蛋白与NFPA的侵袭有关。
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