Dennis J W, Laferté S, Vanderelst I
Division of Cancer and Cell Biology, Mount Sinai Hospital Research Institute, Toronto, Ontario, Canada.
Biochem Soc Trans. 1989 Feb;17(1):29-31. doi: 10.1042/bst0170029.
The expression of beta 1-6-branched complex-type oligosaccharides in several tumour cell models appears to be associated with enhanced metastatic potential. P2B, a major PHA-L-binding glycoprotein was isolated from metastatic MDAY-D2 cells and shown to bind to collagen, fibronectin and laminin with increased affinity after removal of N-linked sialic acid or polylactosamine. Sialylated polylactosamine-containing beta 1-6-branched oligosaccharides on proteins such as P2B and fibronectin may reduce cell adhesion and enhance tumour cell invasion. The loss of branched complex-type oligosaccharides in tumour cells due to somatic mutations or inhibition by swainsonine is also associated with decreased cell proliferation in tissue culture and slower rates of solid tumour growth in mice.
在几种肿瘤细胞模型中，β1-6分支复合型寡糖的表达似乎与转移潜能增强有关。P2B是一种主要的PHA-L结合糖蛋白，从转移性MDAY-D2细胞中分离得到，去除N-连接的唾液酸或多乳糖胺后，它与胶原蛋白、纤连蛋白和层粘连蛋白的结合亲和力增加。P2B和纤连蛋白等蛋白质上含唾液酸化多乳糖胺的β1-6分支寡糖可能会降低细胞黏附并增强肿瘤细胞侵袭。由于体细胞突变或苦马豆素抑制导致肿瘤细胞中分支复合型寡糖的缺失，也与组织培养中细胞增殖减少以及小鼠实体瘤生长速度减慢有关。
