使用凝集素阵列分析正常和致瘤性人乳腺细胞系中的细胞表面碳水化合物表达模式。
Analysis of cell surface carbohydrate expression patterns in normal and tumorigenic human breast cell lines using lectin arrays.
作者信息
Chen Siyuan, Zheng Ting, Shortreed Michael R, Alexander Caroline, Smith Lloyd M
机构信息
Department of Chemistry, McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, Wisconsin 53706, USA.
出版信息
Anal Chem. 2007 Aug 1;79(15):5698-702. doi: 10.1021/ac070423k. Epub 2007 Jun 20.
Abstract
Cell surface carbohydrates play important roles in a wide variety of biological processes including cell adhesion, fertilization, differentiation, development, and tumor cell metastasis. Lectins are proteins of nonimmune origin which recognize and bind to specific carbohydrate structural epitopes. We have recently described the development and use of lectin arrays as tools for the elucidation of the carbohydrate structures expressed on cell surfaces. In the present work this technology is employed for the characterization of differences in carbohydrate expression patterns on normal and tumorigenic human breast cell lines, as well as on sublines differing in their tendency to "home" to different tissues during metastasis. Significant differences were observed, including changes that correlate with metastatic potential as well as with tissue-specific homing of metastatic cells.
摘要
细胞表面碳水化合物在多种生物学过程中发挥重要作用，包括细胞黏附、受精、分化、发育以及肿瘤细胞转移。凝集素是一类非免疫来源的蛋白质，可识别并结合特定的碳水化合物结构表位。我们最近描述了凝集素阵列的开发及应用，它可作为阐明细胞表面表达的碳水化合物结构的工具。在本研究中，该技术用于表征正常和致瘤性人乳腺细胞系上碳水化合物表达模式的差异，以及在转移过程中向不同组织“归巢”倾向不同的亚系上的差异。观察到了显著差异，包括与转移潜能以及转移细胞的组织特异性归巢相关的变化。
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Analysis of cell surface carbohydrate expression patterns in normal and tumorigenic human breast cell lines using lectin arrays.使用凝集素阵列分析正常和致瘤性人乳腺细胞系中的细胞表面碳水化合物表达模式。
Anal Chem. 2007 Aug 1;79(15):5698-702. doi: 10.1021/ac070423k. Epub 2007 Jun 20.
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