Holmer Christoph, Winter Hanno, Nagel Alexandra, Jaenicke Annika, Lauster Roland, Kraft Marc, Buhr Heinz J, Ritz Jörg-Peter, Zickerow Matthias
a Department of General, Visceral and Vascular Surgery , Charité - Universitätsmedizin Berlin, Campus Benjamin Franklin , Berlin ;
b Institute of Engineering Design, Micro and Medical, Department of Medical Engineering , Technische Universität Berlin ;
Int J Hyperthermia. 2016 Aug;32(5):583-6. doi: 10.3109/02656736.2016.1168872. Epub 2016 May 4.
Bipolar radio-frequency-induced thermofusion (BiRTh) of intestinal tissue might replace conventional stapling devices which are associated with technical and functional complications. Previous results of our study group confirmed the feasibility to fuse intestinal tissue using BiRTh-induced thermofusion ex vivo. The aim of this study was now to evaluate the efficacy of fusing intestinal tissue in vivo by BiRTh-induced thermofusion.
In male Wistar rats a blind bowel originating from the caecum was closed either by BiRTh (n = 24) or conventional suture (n = 16). At 6 h, 48 h, 4 days, and 2 weeks after the procedure caecum bursting pressure was measured to compare both groups.
In total 18 of 21 (85.7%) thermofused and 15 of 16 (93.7%) sutured cecal stumps were primarily tight and leakage-proof (p > 0.05). The operative time was comparable in both groups without significant differences. Both groups showed increases in bursting pressure over the post-operative period. The mean bursting pressure for thermofusion was 47.8, 48.3, 55.2, and 68.0 mmHg, compared to 69.8, 51.5, 70.0 and 71.0 mmHg in the hand-sutured group (p > 0.05) after 6 h, 48 h, 4 days, and 2 weeks, respectively.
These results suggest that BiRTh-induced thermofusion is a safe and feasible method for fusing intestinal tissue in this experimental in vivo model and could be an innovative approach for achieving gastrointestinal anastomoses.
肠道组织的双极射频诱导热融合（BiRTh）可能会取代与技术和功能并发症相关的传统吻合器械。我们研究小组之前的结果证实了在体外使用BiRTh诱导热融合来融合肠道组织的可行性。本研究的目的是评估BiRTh诱导热融合在体内融合肠道组织的疗效。
在雄性Wistar大鼠中，通过BiRTh（n = 24）或传统缝合（n = 16）关闭源自盲肠的盲肠段。在手术后6小时、48小时、4天和2周测量盲肠破裂压力，以比较两组。
总共21个经热融合的盲肠残端中有18个（85.7%）、16个经缝合的盲肠残端中有15个（93.7%）最初是紧密且防漏的（p > 0.05）。两组的手术时间相当，无显著差异。两组在术后期间破裂压力均升高。热融合组在6小时、48小时、4天和2周后的平均破裂压力分别为47.8、48.3、55.2和68.0 mmHg，而手工缝合组分别为69.8、51.5、70.0和71.0 mmHg（p > 0.05）。
这些结果表明，在这个体内实验模型中，BiRTh诱导热融合是一种安全可行的融合肠道组织的方法，可能是实现胃肠吻合的一种创新方法。
