Li C-J, Jiang Y-W, Chen S-X, Li H-J, Chen L, Liu Y-T, Gao S, Zhao Y, Zhu X-L, Wang H-T, Wang F-G, Zheng L, Zhou X
College of Animal Sciences, Jilin University, Changchun, China.
Reproductive Medical Center, The Second Hospital of Jilin University, Changchun, Jilin, China.
Andrologia. 2017 Feb;49(1). doi: 10.1111/and.12581. Epub 2016 May 5.
4-Methylcatechol (4-MC) is a potential neuroprotective drug because it stimulates the synthesis of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in neurons. The present study explored the effect of 4-MC on cell growth and testosterone synthesis in the TM3 Leydig cells of mice. 4-MC did not enhance expression of both BDNF and NGF in these cells. However, this compound significantly inhibited cell proliferation and increased the number of apoptotic cells in a dose-dependent manner. The expression profile of Bax/Bcl-2 gene was altered considerably, and mitochondrial activity was significantly decreased in cells. 4-Methylcatechol also inhibited testosterone synthesis in TM3 Leydig cells. The inhibitory roles of this compound in relation to growth and testosterone synthesis in TM3 Leydig cells maybe associated with increased Bax gene expression and decreased mitochondrial activity. As a result, caspase cascade is activated.
4-甲基儿茶酚（4-MC）是一种潜在的神经保护药物，因为它能刺激神经元中脑源性神经营养因子（BDNF）和神经生长因子（NGF）的合成。本研究探讨了4-MC对小鼠TM3睾丸间质细胞生长和睾酮合成的影响。4-MC并未增强这些细胞中BDNF和NGF的表达。然而，该化合物以剂量依赖性方式显著抑制细胞增殖并增加凋亡细胞数量。Bax/Bcl-2基因的表达谱发生了显著改变，细胞中的线粒体活性显著降低。4-甲基儿茶酚还抑制TM3睾丸间质细胞中的睾酮合成。该化合物对TM3睾丸间质细胞生长和睾酮合成的抑制作用可能与Bax基因表达增加和线粒体活性降低有关。结果，半胱天冬酶级联反应被激活。
