百草枯和莠去津对小鼠红白血病细胞自发生长的抑制及诱导分化作用

Inhibition of spontaneous growth and induced differentiation of murine erythroleukaemia cells by paraquat and atrazine.

作者信息

Magnelli L, Fibbi G, Caldini R, Pucci M, Del Rosso M

机构信息

Institute of General Pathology of Florence University, Italy.

出版信息

Food Chem Toxicol. 1989 Feb;27(2):125-8. doi: 10.1016/0278-6915(89)90007-0.

DOI:10.1016/0278-6915(89)90007-0

PMID:2714717

Abstract

The effect of the herbicides paraquat and atrazine on erythroid differentiation has been studied in mouse erythroleukaemic cells. The addition of atrazine or paraquat was shown to inhibit both spontaneous growth and hexamethylene-bis-acetamide (HMBA)-induced differentiation of undifferentiated erythroleukaemic cells. This effects was dose-dependent and occurred at concentrations of less than 10 ppm for both herbicides. Growth inhibition with atrazine (40-45%) was less pronounced than with paraquat (85-90%). Inhibition of differentiation paralleled growth inhibition. A synergistic effect was observed with HMBA, which per se reduced the growth rate of mouse erythroleukaemic cells, and either herbicide. Evaluation of cell viability under all the experimental conditions using either a trypan blue dye exclusion test or labelled chromium indicated that the effects observed were not related to a cytocidal action of atrazine or paraquat.

摘要

已经在小鼠红白血病细胞中研究了除草剂百草枯和莠去津对红细胞分化的影响。结果表明，添加莠去津或百草枯会抑制未分化红白血病细胞的自发生长以及六亚甲基双乙酰胺（HMBA）诱导的分化。这种作用具有剂量依赖性，两种除草剂在浓度低于10 ppm时均会出现此现象。莠去津（40 - 45%）对生长的抑制作用不如百草枯（85 - 90%）明显。分化抑制与生长抑制平行。观察到HMBA（其本身会降低小鼠红白血病细胞的生长速率）与任一种除草剂之间存在协同效应。在所有实验条件下，使用台盼蓝染料排斥试验或标记铬评估细胞活力，结果表明观察到的效应与莠去津或百草枯的杀细胞作用无关。